TIM-3在人非小细胞肺癌CD4^+肿瘤浸润淋巴细胞的表达增强提示预后不良  

Up-regulation of TIM-3 on CD4^+ tumor infiltrating lymphocytes predicts poor prognosis in human non-small-cell lung cancer

作　　者：吉芃[1] 陈迪康 卞建叶 夏瑞[1] 宋心雨[1] 文雯[1] 张学光[1] 朱一蓓[1] 

机构地区：[1]苏州大学医学部基础医学与生物科学学院,江苏苏州215123

出　　处：《细胞与分子免疫学杂志》2015年第6期808-811,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：江苏省大学生创新创业训练计划(201210285039);江苏省自然科学基金面上项目(BK2011289)

摘　　要：目的研究负性共刺激分子T细胞免疫球蛋白和黏蛋白分子3(TIM-3)在人非小细胞肺癌(NSCLC)肿瘤浸润淋巴细胞的表达及临床意义。方法采用免疫荧光标记结合流式细胞术检测56例NSCLC患者肿瘤组织和癌旁组织浸润淋巴细胞表面TIM-3的表达并分析其表达与患者预后的关系。结果 TIM-3在肿瘤浸润CD4+T细胞和CD8+T细胞的表达率分别为(28.64±10.46)%和(30.77±15.58)%,高于癌旁组织的(13.32±6.95)%和(12.98±8.19)%;TIM-3与程序性死亡蛋白1(PD-1)在肿瘤浸润淋巴细胞呈现共表达;TIM-3在肿瘤浸润CD4+T淋巴细胞的表达与NSCLC患者的生存预后有关。结论TIM-3的表达在肿瘤免疫中起到负性调节作用,对患者的生存预后有不良影响。Objective To characterize the expression of negative costimulatory molecule, T cell immunoglobulin and mucin-domain-containing molecules 3 （TIM-3）, on tumor infiltrating lymphocytes （TILs） in human non-small-cell lung cancer （NSCLC） and explore the clinical significance of the expression. Methods A total of 56 human lung cancer tissue specimens were obtained from pathologically confirmed and newly diagnosed NSCLC patients. Infiltrating lymphocytes from both tumor tissues and adjacent normal lung tissues were analyzed for TIM-3 expression by immunofluorescence staining and flow cytometry. Correlation analysis was performed between TIM-3 expression on TILs and the prognosis of the patients. Results The expression of TIM-3 on CD4＋ TILs in tumor tissues [ （28.64 ± 10.46）% ] was significantly higher than that on CD4 ＋ T cells in adjacent normal tissues [ （ 13.32 ± 6.95 ） % ]. Similarly, the expression of TIM-3 on CD8 ＊ TILs in tumor tissues [ （30.77 ± 15.58）% ] was up-regulated as compared with that on CD8 ＋ T cells in adjacent normal tissues [ （12.98 ±8.19）%]. Moreover, majority of the TIM-3 positive TILs from both adjacent normal tissues and tumor lung tissues were positive for another negative costimulatory molecule, programmed death 1 （PD-t）. Importantly, TIM-3 expression on CEH ＋ TILs was correlated with poor prognosis of the patients. Conclusion TIM-3, as a key negative regulator in the anti-tumor immunity, contributes to the tumor immune evasion. It has an adverse influence on the prognosis of NSCLC patients.

关 键 词：负性共刺激分子 TIM-3 非小细胞肺癌 肿瘤浸润淋巴细胞 

分 类 号：R730.3[医药卫生—肿瘤] R734.2[医药卫生—临床医学]