微RNA抑制人舌鳞状细胞癌迁移侵袭能力  

MicroRNA inhibits the migration and invasion of human tongue squamous carcinoma

作　　者：张新华[1] 王庆亮[2] 赵坤[1] 

机构地区：[1]广州医科大学第二附属医院口腔科,510260 [2]中山大学附属第三医院中心实验室

出　　处：《中华生物医学工程杂志》2014年第5期351-356,共6页Chinese Journal of Biomedical Engineering

基　　金：广东省医学科研基金（A2011268）;国家自然科学基金（81302550）

摘　　要：目的研究微RNA-214（miR-214）对舌癌Tca8113细胞迁移侵袭等生物学行为的影响及其起效机制。方法建立Tca8113细胞miR-214模拟物（M）组、阴性对照模拟物（N）组及阴性对照（c）组。采用RT．PCR法检测各组miR-214水平，Westernblot法检测膜型基质金属蛋白酶1（MT1-MMP）、血管内皮细胞生长因子（VEGF）的表达。采用CCK-8实验检测细胞生长抑制率。采用细胞划痕实验和Transwell实验检测抑制细胞侵袭转移的能力。结果与对照组相比，miR-214模拟物干预Tca8113细胞后，M组miR-214表达水平明显升高（P〈0．05），MT1-MMP、VEGF蛋白表达水平降低，细胞迁移侵袭能力明显降低（P〈0．05）。结论miR-214可能通过降低MT1-MMP、VEGF蛋白表达，抑制舌癌Tca8113细胞的迁移侵袭能力。Objective To explore the inhibitory effect and biological mechanism of the micro RNA- 214 （miR-214） on the migration and invasion of human tongue cancer cell line Tca8113. Methods Group M （miR-2 I d mimics）, Group N （negative control mimic） and Group C （negative control） of Tce8113 cells were established. The expression of miR-214 in each group was detected by real-time PCR. The expressions of MT1-MMP and VEGF in each group were detected by Western blot assay. CCK-8 assay was utilized to investigate the rate of cell growth inhibition. And cell migration and invasion were detected by cell scratch test and Transwell assay. Results Compared with the control groups, the miR-214 expression in Group M was significantly increased after Tca8113 cells were transfected by miR-214 mimics （P〈0.05）. Also, the MT1 -MMP and VEGF protein levels in Group M was reduced, along with the significant reduction in cell migration and invasion ability （P〈0.05）. Conclusion miR-214 may inhibit the migration and invasion of tongue cancer cells Tca8113 by down-regulating the expressions of MT1-MMP and VEGF proteins.

关 键 词：舌肿瘤 微RNAS 癌 鳞状细胞 

分 类 号：R739.86[医药卫生—肿瘤]