Periostin在肾细胞癌中的表达情况及其临床意义  

The expression of Periostin in renal cell carcinoma and its clinical significance

作　　者：林建群[1] 陈锡彬[1] 章锐鸿 

机构地区：[1]汕头市第二人民医院泌尿外科,广东汕头515000

出　　处：《现代泌尿外科杂志》2015年第5期329-331,共3页Journal of Modern Urology

摘　　要：目的检测肾细胞癌患者癌组织中Periostin基因表达的改变,探讨其相关的临床意义。方法收集我院2012年12月至2014年3月泌尿外科收治的肾细胞癌患者64例,每例另取癌旁3cm非癌肾组织作为对照。荧光定量PCR检测Periostin基因表达量。结果 Periostin基因在肾癌组织和癌旁正常肾组织中的表达量分别为0.318±0.076和0.142±0.043,癌旁组织中的表达量显著低于肾癌组织(t=5.36,P<0.01)。Periostin基因的表达量在不同的年龄、性别和病理类型之间无显著性差异(P>0.05),高分化患者Periostin基因表达量为0.242±0.051显著低于中、低分化的0.358±0.086(t=2.62,P<0.05),无淋巴结转移患者Periostin基因表达量为0.257±0.057,显著低于有淋巴结转移的0.361±0.082(t=2.51,P<0.05),临床Ⅰ、Ⅱ期患者Periostin基因表达量为0.273±0.053,显著低于临床Ⅲ、Ⅳ期的0.354±0.079(t=2.33,P<0.05)。结论 Periostin基因的异常高表达参与了肾细胞癌的发生发展过程,有可能成为肾细胞癌分级以及预后评估的分子标记物。ABSTRACT：Objective To detect the mRNA expression of Periostin in renal cell carcinoma and to explore its clinical sig‐nificance .Methods A total of 64 patients with renal cell carcinoma treated in our hospital during Dec .2012 and March 2014 were enrolled in this study .The adjacent healthy tissues of each case served as controls .The mRNA expression of Periostin was detected with Real‐time PCR .Results The mRNA expression of Periostin in renal cell carcinoma was 0 .318 ± 0 .076 ,which was significantly higher than that in the healthy tissues （0 .142 ± 0 .043） ,（t=5 .36 ,P0 .05） .The mRNA expression of Periostin in well differ‐entiation group was 0 .242 ± 0 .051 ,lower than that in median and low differentiation group （0 .358 ± 0 .086） ,（ t=2 .62 , P〈0.05） .The mRNA expression of Periostin in non‐lymph node metastasis group was 0 .257 ± 0 .057 ,lower than that in lymph node metastasis group （0 .361 ± 0 .082） ,（t=2 .33 ,P〈0 .05） .The mRNA expression of Periostin in stage I and II group was 0 .273 ± 0 .053 ,lower than that in stage Ⅲ and Ⅳ group （0 .354 ± 0 .078） ,（t=2 .33 ,P〈0 .05） .Conclusion The upregula‐tion of Periostin is involved in the occurrence and development of renal cell carcinoma ,which can be used as a biomarker to es‐timate the differentiation and prognosis of renal cell carcinoma .

关 键 词：肾细胞癌 Periostin基因 荧光定量PCR 

分 类 号：R783.2[医药卫生—口腔医学]