热休克蛋白90α在HBV相关性肝细胞癌中表达的意义  

Expression of heat shock protein 90α in HBV-related hepatocellular carcinoma

作　　者：李慧[1] 李卫华[1] 张志翔[1] 张倩倩[1] 

机构地区：[1]广州医科大学第一附属医院老年病科,广东广州510182

出　　处：《广州医科大学学报》2014年第6期21-24,共4页Academic Journal of Guangzhou Medical University

摘　　要：目的:探讨热休克蛋白90α(HSP90α)在不同转移潜能人肝癌细胞株及HBV相关性肝细胞癌(HCC)患者血清中表达的临床意义。方法:采用Western blotting、ELISA技术检测不同转移潜能肝癌细胞株及HBV相关性肝癌患者、良性肝病患者和健康对照者血清中HSP90α表达水平;回顾性分析HSP90α表达与临床病理资料间的关系。结果:HSP90α在高转移潜能MHCC97H细胞中的表达水平显著高于无转移潜能HepG2细胞(P=0.0002)。肝癌患者及良性肝病患者血清中HSP90α表达水平均明显高于健康对照者(P=0.0001),但肝癌与良性肝病患者间差异无统计学意义(P=0.990)。肝癌患者血清HSP90α表达水平女性高于男性(P<0.05),患者年龄、HBeAg是否阳性、AFP水平、HBV DNA水平及TNM分期均无统计学差异(P>0.05)。结论:HSP90α与HBV相关性HCC转移潜能相关,可望成为HCC侵袭转移、预后评估及区分HBV相关性肝病与健康者的标志物之一。Objective：To investigate the clinical significance of the expression levels of heat shock protein 90α（ HSP90α） in human hepatoma cell lines with different metastatic potentials and the serum of patients with HBV-related hepatocellular carcinoma （HCC）. Methods： Using the Western blotting, enzyme-linked immuno sorbent assay （ ELISA） to measure the expression levels of HSP90α in the hepatoma cell lines with different metastatic potentials, as well as the serum of HBV-related HCC patients, benign liver disease patients and healthy controls. The relationship between the expression of HSP90α and the clinicopathological data was retrospectively analyzed.Results： The expression level of HSP90α in the MHCC97H cells with high metastatic potential was significantly higher than that in the HepG2 cells without metastatic potential （ P=0.0002） . And the mean expression levels of HSP90α in the serum of patients with hepatoma and patients with benign liver disease were significantly higher than that in healthy controls （ P=0.0001） while no statistically significant difference was found between patients with hepatoma and patients with benign liver disease （P=0.990）. And the expression level of HSP90α expression in the serum of patients with hepatoma had no significant correlation with the age of patients, positive HBeAg （ yes/no） , AFP levels,HBV DNA levels and TNM stage but had correlation with the gender （P=0.044）.Conclusion：HSP90α is correlated with the HBV-related HCC metastatic potential, which is expected to become one of the markers of the HCC invasion and metastasis, prognosis assessment, and differentiation between HBV-related liver disease and healthy subjects.

关 键 词：热休克蛋白90Α HBV相关性肝细胞癌 侵袭及转移 

分 类 号：R735.7[医药卫生—肿瘤]