中药合剂对种植体周围骨组织OPG、OPGL、COX-2表达的影响  

Effects of traditional Chinese medicine mixture on the expression of OPG,OPGL and COX-2 in bone tissues around implant

作　　者：陈国栋[1] 蔡嘉喜[1] 黄万仁 梁淑霞[1] 胡超[1] 郭吕华[2] 

机构地区：[1]佛山市顺德第一人民医院口腔科,广东佛山528300 [2]广州医科大学附属口腔医院种植科,广东广州510140

出　　处：《广州医科大学学报》2014年第6期47-50,共4页Academic Journal of Guangzhou Medical University

基　　金：广东省佛山市医学类科技攻关项目(201308234);广东省中医药局立项资助课题(20141182)

摘　　要：目的:探讨中药合剂对种植体周围骨整合的作用机理;方法:健康雄性6m龄新西兰大白兔24只,随机分为对照组(12只)和实验组(12只)。实验组饲料中加入补肾中药合剂,对照组常规饲料。分别喂养3 d后在其胫骨顶端植入纯钛种植体3颗。于种植后30、60、90 d处死相同例数动物(n=6)。免疫组织化学技术检测OPG、PGL、COX-2的表达变化;结果:30 d时实验组OPG、OPGL、COX-2均中度阳性表达,明显高于对照组(弱阳性),两组间有统计学意义(P<0.05);60d时实验组OPG、OPGL表达(强度阳性)明显高于对照组(中强阳性),两组间有统计学意义(P<0.05),而两组COX-2均弱阳性表达,两组间没有统计学意义(P>0.05);90d两组OPG、OPGL、COX-2均弱阳性表达,两组间没有统计学意义(P>0.05);结论:OPG、OPGL和COX-2参与了种植后的骨组织修复过程,而中药合剂能进一步提高种植术后OPG、OPGL和COX-2的表达水平,提示补肾中药合剂可能促进种植体的周围骨整合。Objective：To investigate the mechanism of traditional Chinese medicine （ TCM ） mixture on osseointegration around implant. Methods： Twenty-four healthy male New Zealand rabbits （ 6 months of age ） were randomly divided into the control group （n=12） and the experimental group （n=12）. The fodder in the experimental group was added with kidney-tonifying TCM, and the control group was fed with conventional fodder. All animals were implanted with 3 pure titanium implants respectively at the top of the shin bone after 3 days of feeding. Then, the same number of cases（ n=6） were executed at 30 d, 60 d and 90 d after implantation. And the change in expression of OPG, OPGL and COX-2 were determined by immunohistochemical technique. Results：The expression of OPG, OPGL and COX-2 in the experimental group were moderately positive at 30 d, which were higher than those in the control group （ weakly positive） with statistical significance （ P〈0.05） . The expression of OPG and OPGL in the experimental group （ strongly positive） were significantly higher than those in the control group （moderately strong positive） at 60 d with statistical significance （P〈0.05）. However, the expression of COX-2 in both groups were positive with no statistical significance （ P〉0.05） . All the expression of OPG, OPGL and COX-2 in both groups were weakly positive at 90 d with no statistical significance （P〉0.05）.Conclusion：OPG, OPGL and COX-2 involve in the repair process of bone tissues after implantation, and TCM mixture can further improve the expression levels of OPG, OPGL and COX-2 after implantation, suggesting kidney-tonifying TCM may promote the osseointegration around the implant.

关 键 词：中药合剂 种植 骨整合 OPG/OPGL COX-2 

分 类 号：R783.6[医药卫生—口腔医学]