膀胱癌中锌指结构转录因子1和E钙黏蛋白的表达及其相关性研究  

The expression and correlation of ZEB 1 and E-cadherin in human bladder urothelial carcinoma

作　　者：王一[1] 刘晓强[1] 郭战军[1] 梁博[1] 李常颖[1] 吴长利[1] 刘雨[1] 孙光[1] 

机构地区：[1]天津医科大学第二医院泌尿外科天津市泌尿外科研究所天津市泌尿外科基础医学重点实验室,300211

出　　处：《中华泌尿外科杂志》2015年第5期337-341,共5页Chinese Journal of Urology

基　　金：天津市科委自然基金青年项目（14JCQNJC12700,13JCQNJC11500）

摘　　要：目的 探讨锌指结构转录因子1（zinc finger E-box binding homeobox 1,ZEB1）和E钙黏蛋白在膀胱尿路上皮癌组织和正常膀胱黏膜组织中的表达情况、相关性及其与临床病理特征的关系.方法 收集2008年3月至2011年3月86例膀胱尿路上皮癌患者的手术标本.男51例,女35例.年龄36 ～72岁,平均（64.1±10.2）岁.其中＞65岁40例,≤65岁46例.均经病理检查证实为膀胱尿路上皮癌,病理分级：G128例,G2～G358例.TNM分期：Ta～T1期34例,T2～T4期52例.肿瘤单发49例,多发37例.初发55例,复发31例.16例正常膀胱黏膜组织取自BPH患者开放手术标本.年龄51 ～75岁,平均（58.6±18.4）岁.采用免疫组化染色方法分别检测ZEB1和E钙黏蛋白在膀胱癌组织及正常膀胱黏膜组织中的表达情况,分析两者在膀胱癌组织中表达的相关性,以及两者表达情况与患者临床病理特征的关系.结果 ZEB1在膀胱癌组织和正常膀胱黏膜组织中表达的阳性率分别为39.5％（34/86）和0（0/16）;在高分级（G2 ～ G3）和低分级（G1）膀胱癌组织中表达的阳性率分别为48.3％（28/58）和21.4％（6/28）;在T2～T4期和Ta～T1期膀胱癌组织中表达的阳性率分别为51.9％ （27/52）和20.6％（7/34）,差异均有统计学意义（P＜0.05）.E钙黏蛋白在膀胱癌组织和正常膀胱黏膜组织中表达的异常率分别为61.6％ （53/86）和81.2％（13/16）,差异有统计学意义（P＜0.01）;在高分级（G2 ～ G3）和低分级（G1）膀胱癌组织中表达的异常率分别为53.4％（31/58）和78.6％（22/28）;在T2～ T4期和Ta～T1期膀胱癌组织中表达的异常率分别为51.9％（27/52）和76.5％（26/34）,差异均有统计学意义（P＜0.05）.在膀胱癌组织中,ZEB1的阳性表达与E钙黏蛋白的异常表达间呈负相关（r =0.247,P＜0.05）.结论 ZEB1和E钙黏蛋白在膀胱癌组织中的表达具有负相关性,且两者的表达与膀胱癌的分期、分级密切相关.Objective To explore the expression,correlation and clinic-pathological characters of zinc finger E-box binding homeobox 1 （ZEB1） and E-cadherin in normal bladder tissues and bladder urothelial carcinoma（BUC）.Methods Immunohistochemical staining was performed on paraffin embedded sections to detect the expression of ZEB1 and E-cadherin in 86 cases of BUC,diagnosed by pathology,between March 2008 and March 2011.Among them,51 cases were male and 35 cases were female.The mean age was （64.1 ± 10.2）years,range 36 to 72 years.40 cases were older than 65 years old,and 46 cases were less than 65 years old.The pathological classification included G1 in 28 cases,G2-G3 in 58 cases.The TNM classification showed that Ta-T1 stage in 34 cases,T2-T4 in 52 cases.Single lesion was found in 49 cases and multi lesion was found in 37 cases.55 cases were primary lesion;the other 31 cases were diagnosed as tumor recurrence.Normal bladder mucosa was obtained from 16 patients with BPH.Their age ranged from 51 to 75 years old,mean （58.6 ± 18.4） years old.Similar immunohistochemical staining about ZEB1 and E-cadherin was performed.The expression of ZEB1 and E-cadherin in different tissue and its relationship with the clinic pathological were further analyzed.Results The positive rate of ZEB1 in bladder cancer group （39.5%,34/86） was significantly higher than that in normal bladder mucosa group （0,0/16） （P 〈 0.01）.The positive rate of ZEB1 in high grade bladder cancer （G2-G3） was 48.3% （28/58）,which was significant higher than that in low grade bladder cancer （G1） （21.4%,6/28） （P 〈0.05）;The positive rate of ZEB1 in muscle-invasive bladder cancer （T2-T4） was 51.9% （27/52）,which was significant higher than that in non-muscle-invasive cancer （Ta-T1） （20.6%,7/34） （P 〈 0.01）.The abnormal rate of E-cadherin expression in bladder cancer （61.6%,53/86） was significantly lower than that in normal bladder mueosa （81.2%,13/16） （P 〈 0.01）.The abnormal expression ra

关 键 词：锌指结构转录因子 E钙黏蛋白 膀胱癌 免疫组织化学 

分 类 号：R737.14[医药卫生—肿瘤]