异源表达afsRS_(cla)全局性调控基因提高工业菌株纳他霉素产量  

Improvement of natamycin production in an industrial strain by heterologous expression of the afsRS_(cla) global regulatory genes

作　　者：陶正生 王业民[1] 郑华亮[1] 陶美凤[1] 

机构地区：[1]上海交通大学生命科学技术学院微生物代谢国家重点实验室,上海200030

出　　处：《生物工程学报》2015年第5期744-751,共8页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.31170084);国家高技术研究发展计划(863计划)(No.2010AA10A201)资助~~

摘　　要：前期研究表明棒状链霉菌的全局性调控基因afsRScla异源表达可以激活变铅青链霉菌中两种抗生素的合成。本研究将包含afsRScla基因的质粒pHL851整合到纳他霉素工业生产菌株褐黄孢链霉菌TZ1401的基因组中,使纳他霉素产量提高38%,达到3.56g/L。qRT-PCR检测6个纳他霉素生物合成基因的转录情况,发现其转录水平提高1.9-2.7倍,表明afsRScla通过正调控纳他霉素生物合成基因的转录,从而提高纳他霉素的产量。本研究结果对afsRScla在抗生素工业生产菌株的高产育种应用具有借鉴意义。The afsRScla global regulatory genes from Streptomyces clavuligerus activate the production of two antibiotics in Streptomyces lividans. In this study, we gained an increase of 38% in the production of natamycin （3,56 g/L） in an industrial strain Streptomyces gilvosporeus TZ1401 through the integration of pilL851 that bears the afsRScla global regulatory genes into its genome. We discovered by quantitive real-time reverse transcription PCR （qRT-PCR） that the expression of 6 genes of the natamycin biosynthetic gene cluster were improved from 1.9 to 2.7 times. This suggests that afsRScla improve the production of natamycin through increased transcription. This study provides a good example for applying afsRSda in high yield breeding of industrial antibiotic producers.

关 键 词：afsRScla基因 工业菌株褐黄孢链霉菌TZ1401 纳他霉素 发酵 q RT-PCR 

分 类 号：Q936[生物学—微生物学]