NRP2及miR-486-5p在胃癌中的表达及临床意义  

Expression and clinicopathogical significance of NRP2 and mi R-486-5p in gastric carcinoma

作　　者：连海峰[1] 李明[1] 刘成霞[1] 贾兴芳[1] 李丹[2] 吴小翎[3] 

机构地区：[1]滨州医学院附属医院消化内科,滨州256603 [2]滨州医学院护理学院,滨州256603 [3]重庆医科大学附属第二医院消化内科,重庆400010

出　　处：《重庆医科大学学报》2015年第3期362-366,共5页Journal of Chongqing Medical University

基　　金：山东省高校科技计划资助项目(编号:J11LF75);滨州医学院科研启动基金资助项目(编号:BY2010KYQD02)

摘　　要：目的:检测神经纤毛蛋白-2(neuropilin-2,NRP2)和mi R-486-5p在胃癌组织中的表达,探讨其与胃癌淋巴结转移的关系。方法:收集65例胃癌组织及其配对癌旁正常粘膜组织,免疫组织化学法检测组织中NRP2的表达,real-time PCR检测mi RNA-486-5p的表达,免疫组织化学法检测D2-40标记的微淋巴管密度(microlymphatic density,MLD),统计分析NRP2、mi R-486-5p的表达与MLD相关性。结果:免疫组化提示NRP2在肿瘤中表达增高(78.5%vs.29.2%,χ2=16.046,P=0.000),且与淋巴结转移密切相关(χ2=7.222,P=0.007)。real-time PCR结果显示肿瘤组织mi R-486-5p表达明显下调(0.39±0.16 vs.1.00±0.06,P=0.000),NRP2的表达水平与MLD呈正相关(r=0.384,P=0.015),mi R-486-5p的表达水平与MLD呈负相关(r=-0.755,P=0.000)。结论:NRP2和mi R-486-5p与胃癌发生发展密切相关,有望成为胃癌治疗的新靶点。Objective：To explore the relationship between the expression of neuropilin-2 （NRF2） and microRNA-486-Sp （miR-486- 5p） in gastric carcinoma（GC） and adjacent non-tumorous tissues and lymph node metastasis of GC. Methods：Sixty-five gastric carcinoma and adjacent non-tumorous specimens were collected. Immunohistochemistry（IHC） was used to detect NRP2 protein expression in tissues;quantitative real time PCR was used to detect the expression of miR-486-5p in tissues;microlymphatic density （MLD）was evaluated with lymphatic endothelium-specific marker-D2-40. The relationship between NR.P2,miR-486-Sp and MLD was analyzed. Results：Compared with those in normal gastric mueosa tissues,the expression of NRP2 was significantly up-regulated in GC tissues（P=0.000）,while the expression of miR-486-5p was down-regulated significantly（P=-0.000）. Statistic analysis revealed the expression of mIR-486-5p in GC tissues was negatively correlated with MLD （P〈0.001） and NRP2 was positive correlated with MLD （P=-0.015）. Conclusion：The expression of NRP2 and miR-486-5p is closely associated with lymph node metastasis of gastric carcinoma,indicating that miR-486-5p and NRP2 might be used as targets for molecular therapy of gastric carcinoma.

关 键 词：胃癌 NRP2 miR-486-5p 微淋巴管密度 

分 类 号：R735.2[医药卫生—肿瘤]