LIG4及HSPB1基因单核苷酸多态性与鼻咽癌预后的关系  

The relationship between genetic single-nucleotide polymorphisms of LIG4 and HSPB1 and prognosis in nasopharyngeal carcinomas

作　　者：刘丹[1] 屈艳丽[2] 赵玉霞[1] 于洪[2] 高雅[1] 陈颖[1] 

机构地区：[1]中国医科大学附属第四医院肿瘤放射治疗科,沈阳110032 [2]辽宁省肿瘤医院肿瘤放射治疗三科

出　　处：《中国医师杂志》2015年第5期678-681,共4页Journal of Chinese Physician

基　　金：辽宁省科技厅科学计划资助项目（2011225019）;沈阳市社会发展科技攻关资助项目（F12-193-9-40）;中国医科大学附属第四医院青年创新发展计划基金资助项目

摘　　要：目的 探讨LIG4和HSPB1基因单核苷酸多态性与鼻咽癌预后的关系.方法 采用Taqman real-time PCR对168例鼻咽癌患者外周血DNA进行单核苷酸多态性的基因分型.以单因素和多因素Logistic回归计算比值比（OR）及95％可信区间（CI）表示相对危险度;单变量分析用Long-rank检验,多变量分析用COX比例风险模型.结果 单因素分析及纳入年龄、性别、吸烟状态、饮酒、病理类型及临床分期多因素分析显示LIG4 C/T（rs1805388）和HSPB1 C/G（rs2868371）位点多态性与鼻咽癌复发、转移及总生存无关.结论 LIG4 rs1805388和HSPB1 rs2868371基因多态性与鼻咽癌预后无关.Objective To investigate the relationship between genetic single-nucleotide polymorphisms of ligase Ⅳ （LIG4） and heat shock protein B1 （HSPB1） and prognosis in nasopharyngeal carcinomas.Methods DNAs were extracted by phenol-chlorofrom method from peripheral blood samples of 168 nasopharyngeal carcinoma patients.Genetic single-nucleotide polymorphisms were divided by Taqman realtime polymerase chain reaction （PCR）.All statistical analyses were performed with statistical product and service solutions v13.0.A p-value of less than 0.05 was confirmed as statistical significance.Single-factor and multiple-factor logistic analyses were used to calculate odds ratio （OR） and 95% confidence interval （CI）.Long-rank test and COX were also used.Results No relationship was found between the recurrence,metastasis and overall survival of nasopharyngeal carcinoma and genetic single-nucleotide polymorphisms of LIG4 and HSPB1 with single-factor and multiple-factor logistic analyses.Conclusions LIG4 rs1805388 and HSPB1 rs2868371 had no relationship with prognosis of nasopharyngeal carcinomas.

关 键 词：鼻咽肿瘤/遗传学 DNA连接酶类/遗传学 热休克蛋白质类/遗传学 预后 

分 类 号：R739.63[医药卫生—肿瘤]