沉默叉头盒O类转录因子-3a对卵巢癌SKOV3细胞系肿瘤球形成能力的影响  

Effect of silencing FoxO3a on capability of sphere-formation in ovarian cancer SKOV3 cell line

作　　者：姜玲[1] 曹晓诚[2] 杨剑锋[2] 肖荞 李程[2] 

机构地区：[1]湖南师范大学附属第一医院妇产科,长沙410000 [2]湖南师范大学医学院病理学教研室

出　　处：《中国医师杂志》2015年第5期689-691,694,共4页Journal of Chinese Physician

基　　金：湖南省教育厅科学研究一般资助项目（13C524）

摘　　要：目的 探讨沉默叉头盒O类转录因子-3a（FoxO3a）活性是否影响人卵巢癌SKOV3细胞系肿瘤球形成能力.方法 干细胞条件培养基悬浮培养法得到SKOV3细胞系球形成细胞.FoxO3a特异性siRNA转染SKOV3细胞,Western blot分析FoxO3a磷酸化水平和Bmi1蛋白表达,肿瘤球形成法检测肿瘤球形成率.结果 与亲本细胞相比,SKOV3细胞系球形成细胞具有更高肿瘤球形成率并高表达磷酸化FoxO3a和Bmi1蛋白.FoxO3a特异性siRNA转染抑制FoxO3a蛋白表达,并上调Bmi1蛋白表达,增强肿瘤球形成能力.结论 沉默FoxO3a导致SKOV3细胞系肿瘤球形成能力增强.Objective To explore whether Forkhead O transcription factor-3a （FoxO3a） activity affects the capability of sphere-formation of ovarian cancer SKOV3 cell line.Methods Sphere-forming cells （SFCs） were obtained and amplified through suspended culture with conditioned medium of the stem cells in SKOV3 cell line.After SKOV3 cells were transfected with FoxO3a specific siRNA,the protein expressions of FoxO3a and Bmi1 and the ratio of sphere-formation were compared with Western blot and sphere-forming assay,respectively.Results Compared to parental cells,SFCs from SKOV3 cell line had higher ratio of sphere-formation and over-expressed Bmi1 and pFoxO3a.Transfection of FoxO3a specific siRNA down-regulated the protein expression of FoxO3a and upregulated expression of Bmi1 in SKOV3 cells,and enhanced the capability of sphere-formation.Conclusions Silence of FoxO3a leads to enhanced capability of sphere-formation in SKOV3 cell line.

关 键 词：转录因子/遗传学 基因沉默 RNA 小分子干扰 卵巢肿瘤/治疗/病理生理学/代谢 

分 类 号：R737.31[医药卫生—肿瘤]