RNAi沉默c-Met基因对人脑胶质瘤U251细胞增殖及侵袭能力的影响  

Effect of RNAi silencing c-Met genes on proliferation and invasiveness of human brain U251 glioma cells

作　　者：汪超甲[1] 王辉[1] 胡钧涛[1] 胡胜利[1] 张涛[1] 

机构地区：[1]湖北医药学院附属太和医院神外科,十堰442000

出　　处：《中国临床神经外科杂志》2015年第5期290-292,共3页Chinese Journal of Clinical Neurosurgery

基　　金：湖北省教育厅青年人才项目(Q20112103)

摘　　要：目的探讨RNA干扰技术沉默c-Met基因表达对人脑胶质瘤U251细胞生长活力及体外侵袭能力的影响。方法将设计好的c-Met基因干扰载体转染至U251细胞中,根据转染质粒不同分为空白对照组(不转染任何质粒)、空载体组(转染空载体)和干扰组(转染重组质粒)。采用四唑盐比色法(MTT)检测细胞的生长活力的变化;采用Transwell小室法检测细胞侵袭能力的变化。结果 MTT检测结果显示干扰组光密度值(0.156±0.164)显著低于空白对照组(0.21±0.146;P<0.05)和空质粒组(0.191±0.138;P<0.05),而空质粒组和空白对照组无显著差异(P>0.05)。细胞体外侵袭能力检测结果显示,干扰组穿膜细胞数[(13.60±3.34)个]显著低于空白对照组[(32.90±6.49)个;P<0.05]和空质粒组[(23.10±2.77)个;P<0.05],而空质粒组和空白对照组无统计学差异(P>0.05)。结论沉默c-Met基因表达能明显抑制U251细胞的生长活力及其侵袭能力。Objective To explore the effects of RNA interference （RNAi） c-Met gene on the proliferation and invasiveness of the brain U251 glioma cells. Methods The c-Met interference carrier was transfected into U251 cells in the interference group. The empty plasmid was transfected into the U251 cells in the control group. The changes in the proliferation and invasiveness of U251 cells were determined respectively by MTT and transwell small chamber methods in all the groups. Results MTT method showed that the proliferation of U251 ceils in the interference group decreased to 77.34% of the normal group （P〈0.05）. The transwell chamber method showed that the number of the traswell chamber cells [（13.60±3.34） cells] was significantly less in the interference group than those in the normal group [（32.90±6.49） cells] and empty plasmid group [（23.10±2.77） cells] （P〈0.05）. Conclusions The in vitro proliferation and invasiveness of U251 cells may be significantly inhibited by pGenesil-1-c-Met shRNA through inhibiting the U251 cells c-Met mRNA and protein expressions.

关 键 词：胶质瘤 U251细胞 C-MET基因 RNA干扰 生长活力 侵袭能力 

分 类 号：R739.41[医药卫生—肿瘤] Q789[医药卫生—临床医学]