结核分枝杆菌Rv3519表达与其生物被膜形成能力的研究  

Correlation study between Rv3519 gene expression and biofilm formation ability in Mycobacterium Tuberculosis

作　　者：杨志强[1] 张巧丽[1] 安雅男 王超[1] 孙青松[2] 唐旭东[3] 孟日增[4] 施晓晨 程威[1] 金琨琪[1] 于录[1] 郭娜[1] 

机构地区：[1]吉林大学人兽共患病研究教育部重点实验室/动物医学学院/军需科技学院,吉林长春130062 [2]白城市畜牧科学研究院,吉林白城137000 [3]深圳清华大学研究院创新中药及天然药物研究重点实验室/病毒生物技术国家工程研究中心深圳分中心,广东深圳518057 [4]吉林省出入境检验检疫局,吉林长春130062

出　　处：《扬州大学学报（农业与生命科学版）》2015年第1期1-5,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家"十二五"传染病防治科技重大专项(2012ZX10003002);国家自然科学基金资助项目(31172364;31271951);教育部新世纪人才计划项目(NCET-09-0434;NCET-13-0245);中国博士后科学基金资助项目(2013M530142);深圳市重点实验室建设扶持计划项目(SW201110026);深圳市生物产业发展专项资金重点基础研究项目(JC201005280643A);深圳市药物研发中相关化合物测试与解析高端服务公共技术平台项目(2011-1705);深圳市战略性新兴产业发展专项资金项目(JCYJ20120616142424467)

摘　　要：采用结晶紫染色检测结核分枝杆菌生物被膜的形成能力,实时荧光定量PCR(qRT-PCR)检测生物被膜态的不同结核分枝杆菌Rv3519表达水平,分析Rv3519基因表达与结核分枝杆菌生物被膜形成能力的关系。结果表明:临床分离株产膜能力较H37Rv明显偏低,差异显著(P<0.01);临床分离株之间生物被膜产量差异不显著(P>0.05);生物被膜态的分离株较标准株Rv3519基因表达量偏低,差异显著(P<0.01)。说明结核分枝杆菌生物被膜形成能力与Rv3519基因表达水平相关,Rv3519基因可以作为结核分枝杆菌生物被膜形成能力的潜在分子诊断标志。Biofilm formation ability of Mycobacterium Tuberculosis （M. tuberculosis） was measured by crystal violet staining. Quantitative real time polymerase chain reaction （qRT-PCR） was used to detect the levels of expression of Rv3519 gene in the biofilms of different M. tuberculosis stains. The relationship between Rv3519 gene expression and the level of their biofilm formation ability was explored. Results showed that the ability of biofilm formation of H37Rv was stronger than those of clinical isolates, which was significantly different （P〈0. 01）;there was no significant difference among the biofilm formation ability of clinical isolates（P〉0.05） ; the expression level of Rv3519 of H37Rv was signifi- cantly higher than that of the clinical isolates （P〈0.01）. In short, there was a correlation between the biofilm-formingability and Rv3519 gene expression level of M. tuberculosis strains, and Rv3519 gene can be regarded as a potential molecular diagnostic marker {or bio{ilm-{orming ability of M. tuberculosis.

关 键 词：结核分枝杆菌 生物被膜 实时荧光定量PCR Rv3519 

分 类 号：R378.911[医药卫生—病原生物学]