玉米弯孢叶斑病菌RhoGAP基因家族的全基因组鉴定与生物信息学分析  

Genome-wide Identification and Analysis of RhoGAP Family from Curvularia lunata

作　　者：王玉莹[1] 侯巨梅[1] 赵丰舟 申永强[1] 左豫虎[1] 刘铜[1] 

机构地区：[1]黑龙江八一农垦大学农学院植物病理与应用微生物研究所,黑龙江大庆163319

出　　处：《玉米科学》2015年第3期44-49,共6页Journal of Maize Sciences

基　　金：国家自然基金项目(31101407;31272026;31301611);2013年黑龙江省研究生学术交流项目(黑教研[2013]145号)

摘　　要：RhoGAPs(GTPase activating proteins)通过与Rho GTP酶结合,促使GTP酶水解成Rho GDP无活状态,在生物体中调控着重要的生物学功能。在全基因组上对玉米弯孢叶斑病菌(C.lunata)中RhoGAP基因家族成员进行鉴定,对其序列特征分析。结果表明,玉米弯孢叶斑病菌(C.lunata)有5个RhoGAP基因家族成员,分布在5不同的Scaffold上,其基因结构分析显示,外显子和内含子大小与位置均不一样,表现出复杂的基因结构。序列比对和系统进化分析发现,5个RhoGAP氨基酸序列与其他真菌来源的RhoGAP一样具有特征性保守序列区,与粗糙脉胞菌(N.crassa)和小麦黄斑叶斑病菌(P.tritici-repentis)的RhoGAP蛋白关系密切,其亲缘关系最近。RhoGAPs（GTPase-activating proteins） enhance the RhoGTPases hydrolsis from the GTP-bound Rho proteins to inactive GDP-bound state, causing their inactivation by combined with RhoGTPases, which play an important regulatory function in organism. RhoGAP gene family from Curvularia lunata on genome-wide was identi- fied, and the characteristics of sequences were analyzed. The results showed that five RhoGAP genes distributed in five Scaffolds were identified. All RhoGAP genes had different exon and intron size and location which showed a complex gene structure. Sequence alignment and phylogenetic relationship showed that like other fungi laccase, there was characteristic conserved sequences in each of five RhoGAP amino acid sequence, and phylogenetic analy- sis revealed that RhoGAP genetic relationship closed to Neurospora crassa and Pyrenophora. tritici-repentis. This pa- per will help to study the regulatory function of RhoGAP.

关 键 词：玉米 RHOGAP 弯孢叶斑病菌 基因家族 基因结构 

分 类 号：S513.035.3[农业科学—作物学]