GPC3、AFP在肝细胞肝癌、肝内胆管细胞癌、结节性肝硬化中的表达及鉴别意义  

Expression and diagnosis significance of GPC3 and AFP in hepatocellular carcinoma, hepatic cell carcinoma, nodular cirrhosis

作　　者：辛海荣[1] 栗一帆[1] 马晋峰[1] 高泽峰[1] 

机构地区：[1]山西省肿瘤医院普外二科,太原030013

出　　处：《中国实用医刊》2015年第12期44-46,共3页Chinese Journal of Practical Medicine

摘　　要：目的探讨磷脂酰肌醇蛋白聚糖3（GPC3）、甲胎蛋白（AFP）在肝细胞肝癌、肝内胆管细胞癌、结节性肝硬化的表达，并比较GPC3、AFP在肝细胞肝癌、肝内胆管细胞癌、结节性肝硬化中表达的差异。方法通过对50例原发性肝细胞肝癌、20例肝内胆管细胞癌、22例结节性肝硬化患者采用免疫组织化学方法进行GPC3、AFP检测，比较GPC3、AFP在肝细胞肝癌、肝内胆管细胞癌、结节性肝硬化中表达的差异。结果GPC3在极早期＋早期、中期＋进展期肝细胞肝癌中的阳性率分别为82．4％、84．9％，均明显高于AFP的表达，差异有统计学意义（P〈0．05）。GPC3和AFP两者联合检测可将诊断极早期＋早期、中期＋进展期肝细胞肝癌的敏感性提高为88．2％、87．8％。GPC3在高分化、中分化、低分化肝细胞肝癌中的阳性率分别为75．O％、85．0％、81．8％，均明显高于AFP蛋白的表达，差异有统计学意义（P〈0．05）。GPC3和AFP两者联合检测可将诊断高分化、中分化、低分化肝细胞肝癌中的阳性率的敏感性提高为75．O％、90．0、90．9％。GPC3肝内胆管细胞癌各个分期相比，GPC3在极早期＋早期、中期＋进展期肝内胆管细胞癌中的阳性率分别为0％、6．25％，与AFP蛋白在肝内胆管细胞癌中的表达比较，差异未见统计学意义（P〉0．05）。GPC3在高分化、中分化、低分化肝细胞肝癌中的阳性率分别为25．O％、0、0，与AFP蛋白在肝内胆管细胞癌中的表达，差异未见统计学意义（P〉0．05）。结论GPC3高表达于肝细胞肝癌组织中，对早期肝细胞肝癌诊断、区分良性及恶性早期占位性病变有重要提示作用。Objective To investigate the expression of GPC3 and AFP in hepatocellular carcino- ma, intrahepatic cholangiocarcinoma, cirrhosis, and to compare the differences of expression of GPC3 and AFP in hepatocellular carcinoma, intrahepatic cholangiocarcinoma, nodular cirrhosis. Methods GPC3 and AFP levels were detected by immunohistochemistry method in 50 cases of primary hepatoceUu- lar carcinoma, 20 cases of intrahepatic cholangiocarcinoma and 22 cases of nodular cirrhosis. The differ- ences of expression of GPC3 and AFP in hepatocellular carcinoma, intrahepatic cholangiocarcinoma and nodular cirrhosis were compared. Results The positive rate of GPC3 in the very early and early, inter- im and advanced hepatocellular carcinoma was 82. 4%, 84. 9% respectively, they were significantly higher than the expressions of AFP protein, the differences were statistically significant （P 〈 0. 05 ）. The sensitivity of combined detection of GPC3 and AFP in very early and early, interim and advanced stage of hepatocellular carcinoma increased to 88.2%, 87.8% respectively. The positive rate of GPC3 in high differentiation, differentiation, poorly differentiated hepatocellular carcinoma were 75.0%, 85.0%, 81.8%, they were significantly higher than the expression of AFP protein, the differences were statisti- cally significant （P 〈 0. 05 ）. The positive rate sensitivity of combined detection of GPC3 and AFP in- creased to 75. %, 90. 0%, 90. 9% in diagnosis of high differentiation, differentiation, poorly differentia- ted hepatocellular carcinoma. Compared with each stage of intrahepatic cholangiocarcinoma, the positive rate of GPC3 in the very early and early period, interim and advanced stage was 0, 6. 25 % respectively, there were no significant differences compared with the expression of AFP protein in intrahepatic cholan- giocarcinoma（P 〉 0. 05 ）. The positive rate of GPC3 in high differentiation, differentiation, poorly differ- entiated intrahepatic cholangiocarcinoma were 25%, 0, 0, and there were no significant

关 键 词：肝细胞肝癌 肝内胆管细胞癌 结节性肝硬化 磷脂酰肌醇蛋白聚糖3 甲胎蛋白 

分 类 号：R735.7[医药卫生—肿瘤]