浙江省义乌市鼠类中耶尔森菌调查与分析  

Investigation and analysis on Yersinia in rodent populations in Yiwu,Zhejiang province

作　　者：陈劲华[1] 翁正军 朱志宏[1] 陈波[1] 楼挺 石国祥[2] 

机构地区：[1]义乌市疾病预防控制中心微生物检验科,浙江义乌322000 [2]浙江省疾病预防控制中心,杭州310051

出　　处：《中国媒介生物学及控制杂志》2015年第3期262-265,共4页Chinese Journal of Vector Biology and Control

基　　金：卫生行业科研专项(201202021)~~

摘　　要：目的调查浙江省义乌市鼠疫疫源地啮齿动物中致病性耶尔森菌的分布情况,并分析菌株的生物学特征。方法采集鼠粪便进行耶尔森菌分离,对分离获得的菌株进行生物分型、血清分型和毒力基因鉴定。结果 2005—2013年共检测3623份标本,分离到小肠结肠炎耶尔森菌74株,假结核耶尔森菌11株;耶尔森菌的检出率以春季最高(5.72%)。68株小肠结肠炎耶尔森菌有生物1A型(55株)、生物3型(1株)2个型别;血清分型O:5血清型有8株、O:8血清型有2株;毒力基因检测有29株菌检出ystB基因。结论义乌市鼠疫疫源地内未检出鼠疫菌,但分离出假结核菌和小肠结肠炎菌;在鼠疫监测同时也对其他两种致病性耶尔森菌开展监测和相关研究,对鼠疫的监测有指导意义。Objective To understand the distribution of the other pathogenic Yersinia in rodents in the plague focus of Yiwu, Zhejiang province and analysis of biological characteristics of the strains. Methods Yersinia was isolated from mouse feces, the isolated strains were identified by biological typing, serum typing and virulence gene detection. Results In total, 3623 samples were screened from 2005 to 2013, 74 strains of Yersinia enterocolitica, and 11 strains of Y. pseudotuberculosis were isolated, the highest detection rate of Yersinia was in the spring （5.72%）. Of the 68 strains of Y. enterocolitica, biotype 1A （55 strains）, biotype 3 （1 strain）, serotype 0 ： 5 （8 strains） and serotype 0 ： 8 （2 strains） were identified, ystB gene was detected from 29 strains. Conclusion The Y. pestis has not detected in the plague focus in Yiwu, but the Y. enterocolitica and Y. pseudotuberculosis were isolated. It is necessary to correlate study of other pathogenic Yersinia in plague surveillance.

关 键 词：啮齿动物 小肠结肠炎耶尔森菌 假结核耶尔森菌 血清型 毒力基因 

分 类 号：S443[农业科学—农业昆虫与害虫防治] R378[农业科学—植物保护]