东南沿海地区汉坦病毒的遗传进化分析  

Phylogenetic analysis of Hantavirus in the South-east coastal area of China

作　　者：李婵[1] 姚苹苹[1] 朱函坪[1] 徐芳[1] 杨章女[1] 岳明[2] 谢荣辉[1] 孙一晟[1] 徐致遥 王长军[4] 张云[4] 

机构地区：[1]浙江省疾病预防控制中心浙江省肾综合征出血热疫苗研究重点实验室,杭州310051 [2]南京医科大学第一附属医院 [3]温州医科大学 [4]南京军区军事医学研究所

出　　处：《中国媒介生物学及控制杂志》2015年第3期275-278,共4页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(81171609;81473036)~~

摘　　要：目的对浙江、福建省及上海市东南沿海地区汉坦病毒的基因进行比较。方法收集浙江、福建省及上海市汉坦病毒部分M基因序列，应用Mega4．0和DNAStar软件包进行系统发生分析，以邻位相连法（NJ）构建系统发生树，分析东南沿海地区浙江、福建省及上海市汉坦病毒的基因差异，分析采用1000个多序列组。结果经对浙江、福建省及上海市若干株汉坦病毒基因的序列比较与系统进化分析，浙江省分离的同型别毒株的基因序列同源性高于福建省与上海市的分离毒株，而浙江省同地区分离株的核苷酸同源性更高，地区接近的分离株系统进化分支的分布也大部分更靠近。汉滩型病毒（HTNV）福建株的ZH53和汉城型病毒（SEOV）浙江建德株Gou3和ZJ5与本研究同型别的其他株及国际标准株的核苷酸同源性较低，分别为82．7％～86．3％和84．0％～85．3％，而且分别在HTNV和SEOV发生群中构成一独立分支。浙江省从田鼠中分离到SEOV，产生宿主“溢出”现象。结论浙江、福建省及上海市东南沿海地区汉坦病毒的基因差异和亲缘远近关系主要表现在地区性，显示出高度的地理聚集现象，浙江省建德地区和福建省分别存在SEOV和HTNV的特殊亚型病毒。Objective To analyze the sequence of Hantavirus （HV） from Zhejiang, Fujian provinces and Shanghai municipal. Methods The sequence of M segment of several HV strains from coastal areas of Zhejiang, Fujian province and Shanghai municipal were collected and analyzed by Mega 4.0 and DNAStar software, phylogenetic trees were built by neighbor joining method （NJ） and analyzed to compare the similarity of HV strains isolated from Zhejiang, Fujian provinces and Shanghai municipal. Results The phylogenetic tree revealed that the similarity of isolates in the same province is much higher than the other, meanwhile, in same province or city, the nucleotide similarity of the same region is higher than the other regions. The distributions of the isolates from closer regions share higher sequence similarity in the phylogenetic branches. The ZH53 strain of HTN isolated from Fujian province, Gou3 strain and ZJ5 strain of SEO isolated from Jiande region in Zhejiang province formed a distinct phylogenic lineage in HTNV clade and SEOV clade respectively, and these strains were different from other variants and international standard strains with the similarity of 82.7%-86.3% and 84.0%-85.3% respectively. The SEOV strains in Zhejiang were isolated from field mouse indicating the phenomenon of ＂host spillover＂. Conclusion The sequence similarity and the phylogeny of HV in southeast costal area of Zhejiang, Fujian provinces and Shanghai is closed related to the isolated regions, indicating geographic distribution of HV.

关 键 词：汉坦病毒 序列分析 系统进化 地理聚集现象 特殊亚型 

分 类 号：R373.3[医药卫生—病原生物学]