熟三七对Lewis肺癌的抑制及对联合用药的增效减毒作用研究  

Inhibition effect of steamed Panax notoginseng on Lewis lung cancer and the “effect-enhancing and toxicity-reducing effect”when use in combination with chemotherapy drugs

作　　者：何宜航 杨桂燕[1] 阳向波 邹俊波[1] 纪奇森 张帅杰 杨明[2,4] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]四川联成迅康医药股份有限公司,成都610000 [3]成都百草和济科技有限公司,成都610000 [4]江西中医药大学制剂教育部重点实验室,南昌330004

出　　处：《世界中医药》2015年第5期664-670,共7页World Chinese Medicine

摘　　要：目的：考察熟三七粉对Lewis肺癌的抑制作用及与紫杉醇、培美曲塞二钠联合用药的增效减毒作用。方法：首先将接种Lewis肺癌的C57 BL／6小鼠按体重随机分为模型组，阳性组，生、熟三七低、中、高剂量组，每组13-14只。其中生、熟三七粉按低、中、高剂量（585 mg·kg－1，1170 mg·kg－1，2340 mg·kg－1）连续灌胃15 d，阳性组以60 mg·kg －1剂量于第1天腹腔注射CTX 1次，试验结束后计算抑瘤率，并统计给药前后体重、体重比值（ FBW／IBW）、死亡情况；再将新接种Lewis肺癌的C57BL／6小鼠按体重随机分为9组，每组10只，即模型组（0．5％ CMC－Na溶液）、熟三七组、生三七组、紫杉醇组、熟三七联合紫杉醇组、生三七联合紫杉醇组、ALIMTA组、熟三七联合ALIMTA组、生三七联合ALIMTA组。生、熟三七以1170 mg·kg－1连续灌胃给药15 d，紫杉醇（10 mg·kg－1）、ALIMTA（120．3 mg·kg－1）自给药第1天起每3 d腹腔注射1次，共3次。试验结束后计算抑瘤率，测定外周血象，并考察给药前后体重、体重比值（ FBW／IBW）、死亡情况。结果：与模型组相比，熟三七（1170 mg·kg－1）具有极显著抑瘤率41％（P＜0．01）、生三七（2340 mg·kg－1）具有显著抑瘤率（P＜0．05），但无临床意义，且表现出一定不良反应；生、熟三七与紫杉醇、ALIMTA连用均能提高抑瘤率，且熟三七表现出降低紫杉醇毒副作用的趋势，而生三七却表现出一定不良反应。结论：熟三七能显著抑制Lewis肺癌生长，与化药联合使用可表现出较好协同作业用，并可拮抗紫杉醇毒副作用；生三七对肿瘤生长的抑制不具有临床意义，虽在联合用药中表现出较好的协同作用，但不良反应明显。Objective：To Investigate the inhibition effect of steamed Panax notoginseng on Lewis lung cancer , and find out wheth-er there is a effect-enhancing and toxicity-reducing effect when use in combination with paclitaxel or pemetrexed disodium .Meth-ods：C57 BL/6 mice inoculated with Lewis lung cancer were randomly divided into model group , positive group , raw Panax notog-inseng low, medium and high dose group , steamed Panax notoginseng low , medium and high dose group （ each group n =13-14）.Among them, raw and steamed Panax notoginseng groups received intragastric administration according to low , middle, high dose（585 mg·kg -1, 1170 mg·kg-1, 2340 mg·kg-1）, positive group mice received intraperitoneal injection of CTX （60 mg？kg -1 ） on the first day.After the trial, calculated inhibition rate against tumor and recorded body weight before /after administra-tion, body weight ratio（FBW/IBW） and death.Then the new Lewis lung cancer inoculated C57BL/6 mice were randomly divided into 9 groups （n =10）, model group （0.5% CMC-Na solution）, steamed Panax notoginseng group, raw Panax notoginseng group, paclitaxel group, steamed Panax notoginseng plus paclitaxel group , raw Panax notoginseng plus paclitaxel group , ALIMTA group, steamed Panax notoginseng plus ALIMTA group and raw Panax notoginseng plus ALIMTA group .Steamed and raw Panax notoginseng were continuously intragastric administrated at the dose of 1170 mg·kg -1, and paclitaxel（15 d, 10 mg·kg -1）, AL-IMTA （120.3 mg·kg -1） were intraperitoneal injected since Day 1（every three days, 3 times in total）.After the trial, calculated inhibition rate against tumor , determined peripheral hemogram and recorded body weight before /after administration , body weightratio（FBW/IBW） and death.Results： Compared with the model group, steamed Panax notoginseng（1170 mg·kg-1） showed significantl inhibition rate（41%, P〈0.01）;raw Panax notoginseng（2340 mg·kg-1） also showed significantl inhibition rate （P〈0.05 ）

关 键 词：熟三七 LEWIS肺癌 抑制 联合用药 增效减毒 

分 类 号：R734.2[医药卫生—肿瘤]