五爪金龙叶总黄酮的提取、纯化及抗氧化活性研究  

Extraction,purification and antioxidant activity of total flavonoids from leaves of Ipomoea cairica(linn.)sweet

作　　者：易运红[1] 吕君亮[1] 刘淑娜[1] 吴功庆[1] 

机构地区：[1]广东药学院医药化工学院,中山528458

出　　处：《中国食品添加剂》2015年第5期86-92,共7页China Food Additives

基　　金：广东省大学生创新创业训练项目(1057313023)

摘　　要：采用超声辅助提取五爪金龙叶总黄酮,在单因素试验基础上,利用正交试验优化提取黄酮的最佳工艺条件,粗黄酮经大孔树脂HPD600纯化,通过测定精制黄酮和抗坏血酸的还原力、羟自由基消除能力和DPPH自由基的清除能力,来评价其抗氧化活性。结果表明,五爪金龙叶总黄酮的最佳提取条件为温度50℃,乙醇浓度80%,料液比1∶35(g·m L-1),时间15min,超声功率120W,总黄酮的提取率可达3.613%。大孔树脂纯化后黄酮含量由18.53提高至83.62%。五爪金龙叶纯化黄酮的还原能力、羟自由基消除能力比同浓度的抗坏血酸差,但随着浓度增大而增大;DPPH消除能力和同浓度抗坏血酸相仿,五爪金龙叶黄酮具有一定抗氧化活性,可作为天然抗氧剂开发。The ultrasound-assisted extraction conditions of flavonoids from Leaves of Ipomoea cairica （Linn.） Sweet were optimized by using orthogonal test design. The crude extraction was then purified by HPD600 macroporous adsorption resin. The antioxidant activities of the purified flavonoids and vitamin C were evaluated in vitro by hydroxyl free radical, DPPH radical scavenging capacity and reducing power, respectively. The results indicated that the highest extraction yield of flavonoids using the ultrasound-assisted extraction could obtain up to 3.613% under the following conditions ： ethanol concentration of 80% （v/v） as solvent, ultrasonic power at 120 W and liquid to solid ratio of 1 ： 35 （g · mL-l）, extraction for 15 rain at the temperature of 50 ℃ . The flavonoid content increased from 18.53% to 83.62 % after macroporous resin purification. Reduction capability and the hydroxyl radical scavenging ability were weaker than the same concentration of vitamin C. But as the concentration increases, reduction capability and the scavenging abliliby increases. They have similar DPPH radical elimination ability. Flavonoids from Leaves of Ipomoea cairica （Linn.） Sweet have some antioxidant capacity and can be used as a source of potential antioxidant.

关 键 词：五爪金龙叶 黄酮 工艺优化 大孔树脂 抗氧化 

分 类 号：TS202.1[轻工技术与工程—食品科学]