灵芝多糖联合二甲双胍对2型糖尿病模型大鼠氧化应激的影响  

Effect of Ganoderma Lucidum Polysaccharides Combined with Metformin on Oxidative Stress of Type 2 Diabetic Rats in Vivo

作　　者：乔进[1] 窦志华[1] 施忠[1] 吴锋[2] 孟国梁[2] 陈惠[3] 郑惠华[3] 

机构地区：[1]江苏省南通市第三人民医院药剂科,南通226001 [2]南通大学医学院药理学系,南通226001 [3]江苏安惠生物科技有限公司,南通226006

出　　处：《医药导报》2015年第6期718-721,共4页Herald of Medicine

基　　金：科技部十一五国家科技支撑计划子课题项目(2006DAI06A20-02);江苏省优势学科项目

摘　　要：目的研究灵芝多糖联合二甲双胍对2型糖尿病大鼠氧化应激的影响。方法采用斯泼累格·多雷(SD)大鼠高脂饮食4周后,腹腔注射小剂量链脲佐菌素30 mg·kg-1造模。成模后将大鼠随机分为模型对照组、灵芝多糖组(给予灵芝多糖600 mg·kg-1)、二甲双胍组(给予二甲双胍600 mg·kg-1)及联合用药组(给予灵芝多糖300 mg·kg-1+二甲双胍300 mg·kg-1),另设正常对照组。给药治疗12周末测量大鼠空腹血糖;测定血清超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、总胆固醇(TC)、三酰甘油(TG)水平。结果用药组空腹血糖水平均低于模型对照组(P<0.01),其中联合用药组血糖显著低于单独用药组(P<0.01)。与模型对照组比较,用药组血清TC、TG水平明显下降(P<0.05或P<0.01),与单独用药组比较,联合用药组血清TC、TG水平明显降低(P<0.05或P<0.01);与模型对照组比较,用药组血清SOD水平明显升高(P<0.01);与单独用药组比较,联合用药组血清SOD水平明显升高(P<0.05)。与模型对照组比较,用药组血清MDA水平明显降低(P<0.01),联合用药组血清MDA较单独用药组明显降低(P<0.05)。与模型对照组比较,用药组血清CAT、GSH-Px水平明显升高(P<0.05或P<0.01),联合用药组血清CAT、GSH-Px水平较单独用药组明显升高(P<0.05)。结论灵芝多糖、二甲双胍联合用药能够有效对抗2型糖尿病大鼠体内氧化应激作用,效果优于单独用药,其机制可能与增强体内SOD、CAT、GSH-Px水平及调节血脂障碍有关。Objective To study the effect of ganoderma lucidum polysaccharides combined with metformin on oxidative stress of type 2 diabetic rats. Methods SD rats were fed with high fat diet for 4 weeks and injected with streptozotocin （30 mg·kg-1 ） to produce type 2 diabetic model. The diabetic rats were randomly divided into diabetes model group, ganoderma lucidum polysaccharides group （600 mg·kg-1 ）, metformin group （600 mg·kg-1 ）, combination group （ganoderma lucidum polysaccharides 300 mg·kg-1＋ metformin 300 mg·kg-1 ）, After 12 weeks of treatment, the level of fasting blood glucose was determined, and the activity of superoxide dismutase （ SOD）, malondialdehyde （ MDA）, catalase （ CAT）, glutathione peroxidase （GSH-Px）, total cholesterol （TC） and triglyceride （TG） were detected. Results The levels of fasting blood glucose in the treatment groups were significantly lower than that in the diabetes model group （P〈0. 01）. Furthermore, fasting blood glucose in the combination group was significantly lower than that in ganoderma lucidum polysaccharides group and metformin group （P〈0. 01）. Compared with diabetes model group, serum TC and TG in the treatment groups were significantly lower （P〈0. 05, P〈0. 01）. Serum TC and TG were significantly lower in the combination group than in ganoderma lucidum polysaccharides group and metformin group （P〈0. 05, P〈0. 01）. Compared with diabetes model group, serum SOD levels in the treatment groups were significantly higher （P〈0. 01）. Compared with ganoderma lucidum polysaccharides group and metformin group, serum SOD levels in the combination group was significantly higher （P〈0. 05）. Compared with diabetes group, serum MDA levels in the treatment groups were significantly lower （P〈0. 01）. Serum MDA in the combination group was significantly lower than that in ganoderma lucidum polysaccharides group and metformin group （ P〈0. 05）. Compared with diabetes model group, serum CAT and GSH-Px in the

关 键 词：灵芝多糖 二甲双胍 糖尿病 氧化应激 

分 类 号：R977.15[医药卫生—药品]