木霉Tr-92菌株厚垣孢子发酵条件的优化  

Optimization of liquid fermentation conditions for production of Trichoderma Tr-92 chlamydospores

作　　者：张晶晶[1,2] 黄亚丽[1] 马宏[1] 贾振华[1] 史延茂[1] 宋水山[1] 

机构地区：[1]河北省科学院生物研究所,河北省主要农作物病害微生物控制工程技术研究中心,石家庄050081 [2]河北工业大学化工学院,天津300130

出　　处：《植物保护》2015年第3期25-29,共5页Plant Protection

基　　金：河北省自然科学基金(C2010001565);河北省省级科技计划专项工作类项目(13256504D)

摘　　要：利用单因素试验、正交试验方法对木霉Tr-92厚垣孢子发酵培养基及培养条件进行优化,筛选获得了适合此菌株厚垣孢子产生的最佳培养基组成为:草炭2.5%,玉米浆4.5%,葡萄糖2%,酵母膏0.5%;最佳培养条件为:接种量3%,装液量75mL/250mL,转速为180r/min,初始pH 5.0,温度28℃,培养时间7d。在此培养条件下,木霉Tr-92菌株厚垣孢子产量达到3.01×108个/mL。与优化前相比,厚垣孢子产量增长130.80%。通过培养条件优化确定了适合木霉Tr-92菌株产生厚垣孢子的条件,为高效木霉厚垣孢子生防菌剂的研制奠定了基础。The optimal liquid fermentation conditions for production of Trichoderma Tr-92 chlamydospores were investigated by single factor test and orthogonal test. The results indicated that the optimal conditions for produc- tion of Trichoderma Tr-92 chlamydospores were as followed.. 2.5% peat, 4.5% maize steep liquor, 2% glucose, 0.5% yeast extract, 3% inoculum, 180 r/min, 28 ℃, initial pH 5.0, and 75 mL liquid medium per 250 mL flask. The actual yield of chlamydospores was 3.01 ×10^8spores/mL after 7 days. The chlamydospore production increased by 130.80% compared with the control.

关 键 词：木霉 厚垣孢子 液体发酵 培养基 发酵条件 

分 类 号：Q949.2[生物学—植物学] S476[农业科学—农业昆虫与害虫防治]