耐高温酒精酵母的筛选及26SrDNA序列鉴定  

Isolation of High Temperature Resistant Alcohol Yeast and Its Identification of 26SrDNA Sequence

作　　者：李艾[1] 

机构地区：[1]唐山学院环境与化学工程系,河北唐山063000

出　　处：《唐山学院学报》2015年第3期76-79,82,共5页Journal of Tangshan University

摘　　要：将从不同地点采集到的样品分离后得到127株酵母菌,再经过四级筛选得到1株耐高温酒精酵母,将其命名为A27,利用26SrDNA的D1/D2区域序列分析法对其进行分子鉴定,结果显示与报道的酿酒酵母26SrDNA同源性为99%,由此在分子水平上验证了A27菌株为酵母属的酿酒酵母。The author of this paper separated the samples collected from different locations, got 127 yeast strains, then obtained 1 high-temperature alcohol yeast strain after 4 screenings, which was named A27, and finally made a molecular identification of it by using 26 srdna D1/D2 area sequence analysis method. The results reveal that it has 99% homology of Saccharomyces cerevisiae 26SrDNA, thus proving that the strain A27 is saccharomyces cerevisiae at the molecular level.

关 键 词：高温 酒精酵母菌 分离筛选 鉴定 

分 类 号：Q935[生物学—微生物学]