染料木素抑制人胰腺癌细胞株PANC-1细胞增殖机制的研究  

Genistein inhibits proliferation of PANC-1 human pancreatic cancer cells

作　　者：马志亮[1] 崔啸晨 李夏青[2] 王丽凤 苑洪莲[4] 卫建平[5] 安峡[6] 尹彩虹[6] 王志茹[1] 

机构地区：[1]山西医科大学第二医院普外科,太原030001 [2]山西医科大学病理生理教研室,太原030001 [3]山西大医院病理科,太原030001 [4]山西医科大学第二医院手术室,太原030001 [5]山西医科大学第二医院病理科,太原030001 [6]朔州市中心医院内科,朔州036000

出　　处：《中华细胞与干细胞杂志（电子版）》2015年第2期43-47,共5页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：山西省归国留学项目(2010099)

摘　　要：目的探讨不同浓度染料木素对胰腺癌细胞作用及其抑癌基因表达的研究。方法本实验共分实验组(80,120,160,200μmol/L)的染料木素、空白对照组(PBS)和标准对照(吉西他滨)。各组培养基处理细胞12,24,36,48,60 h后,细胞计数试剂盒(CCK-8)法检测人胰腺癌细胞株PANC-1在染料木素作用下的增殖活性;流式细胞术检测人胰腺癌细胞株PANC-1在染料木素木素作用下的凋亡细胞百分率;用逆转录-聚合酶连反应(RTPCR)方法测定P53及P21抑癌基因的表达;Western-blot法检测抑癌基因P53和P21的蛋白表达水平。细胞凋亡与细胞基因mRNA采用的是LSD法,细胞增殖数据采用的是配对t检验。结果流式细胞术显示,人胰腺癌细胞株PANC-1处理后60 h时在不同浓度(80,120,160,200μmol/L)的染料木素作用下细胞凋亡率分别为(3.72±1.58)﹪、(35.98±3.76)﹪、(51.36±4.32)﹪和(64.10±2.09)﹪。吉西他滨(gemcitabine,GEM)组为(43.45±5.28)﹪,不同浓度染料木素组与标准对照组间比较差异有统计学意义(均P<0.01);细胞增殖效果:160μmol/L与200μmol/L、120μmol/L与200μmol/L、200μmol/L与吉西他滨组之间比较,均有统计学意义(P<0.01),而160μmol/L与吉西他滨组比较无统计学意义(P>0.05);Western-blot检测蛋白水平显示160μmol/L和200μmol/L染料木素相对于空白对照能够明显的增强抑癌基因P21和P53的蛋白水平的表达,相对于标准组没有明显的差异。结论染料木素可以抑制人胰腺癌细胞株PANC-1增殖,并且初步探索了这种抑制作用表现出浓度及时间依赖性。Objective To explore the effects of Genistein on pancreatic cancer and the expression of tumor suppressor genes. Methods This study included experimental group （80, 120, 160, 200μmol/L Genistein）, positive control group （gemcitabine） and blank group （PBS）. At the different time points （12, 24, 36, 48 and 60 h）proliferation of human pancreatic cancer cell line PANC-1 was evaluated with CCK-8 assay. Apoptotic cells were detected with FCM. Exoress of tumor suppressor genes P53 and P21 was evaluated using RT-PCR. Protein expression level of P53 and P21 was evaluated using Western-blot. The statistical analysis of apoptotic apoptotic and cell mRNA was the way of LSD. The statistical analysis of the proliferation activity was t test of paired sample. Results The percentages of apoptotic pancreatic cancer cells were （3.72 ± 1.58） %, （35.98 ± 3.76） %, （51.36 ± 4.32） % and （64.10 ± 2.09） % 60 h after treatment with different concentrations of Genistein. The percentages of apoptotic pancreatic cancer cells was 43.45 ±5.28 for the gemcitabine group （P 〈 0.05）. Cell proliferation wase significant different between the 160 μmol/L Genistein and 200 ~tmol/L Genistein, 120 μmol/L Genistein and 200 μmol/L Genistein, 200 ~tmol/L Genistein and gemcitabine （P 〈 0.05） ; however there was no significant difference between 160 μmol/L Genistein and gemcitabine （P 〉 0.05 ）. Genistein at 160 μmol/L and 20 μmol/L could obviously increase the protein expression of tumor suppressor genes P53 and P21 compared with the blank control group （P 〈 0.01 ）, however, they are not significantly different with the gemcitabine group （P 〉 0.05 ）. Conclusion Genistein can inhibit the proliferation of human pancreatic cancer cell line PANC-1.

关 键 词：胰腺肿瘤 染料木素 吉西他滨 细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤]