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作 者:杨庆珍[1,2] 郑司浩[2] 黄林芳[2] 田义新[1]
机构地区:[1]吉林农业大学中药材学院,长春130118 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193
出 处:《世界科学技术-中医药现代化》2015年第3期723-728,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基 金:国家自然科学基金委面上项目(81274013):西洋参不同产地品质相关"地理特异基因群"的发掘;负责人:黄林芳;国家自然科学基金委面上项目(81473315):地理格局及生态驱动揭示肉苁蓉品质生态型机理;负责人:黄林芳;国家自然科学基金委重点项目(81130069):道地药材形成的生物学实质;负责人:黄林芳
摘 要:目的:电子鼻技术和化学成分分析对不同生长年限黄芪样品进行准确、快速鉴别分析。方法:建立电子鼻对2年、7年和10年以上生长年限黄芪样品的响应特点分类模型,应用主成分分析(PCA)和判别因子分析(DFA)对不同生长年限的黄芪进行分类。主成分分析法研究不同生长年限黄芪样品间化学成分和品质的差异性。结果:2年、7年和10年以上生长年限黄芪样品组内电子鼻检测结果主成分分析和判别因子分析均可分为一类。相同生长年限黄芪化学成分含量相似,不同生长年限黄芪化学成分含量差异明显。结论:化学成分分析结果揭示黄芪生长年限的鉴别对于黄芪质量控制具有重要意义。电子鼻技术能够实现对不同生长年限黄芪样品快速、灵敏、无损地鉴别,在中药材的质量控制方面具有重要的应用价值。In this article, the method of quick and accurate identification of Astragalus membranaceus (Fisch.) Bge. vat. mongholicus (Bge.) Hsiao in different growth years by electronic nose (e-nose) technique and chemical analysis was developed. An e-nose was used to detect the odors of A. membranaceus, var. rnongholicus samples in two-growth-year, seven-growth year and more than a decade growth years for establishing the classification model of response characteristics. Principal component analysis (PCA) and discriminant factor analysis (DFA) were performed to differentiate the A. membranaeeus, var. mongholicus samples in different growth years. PCA was performed to investigate the difference in the chemical composition and quality of different growth years in A. membranaceus, var. mongholicus samples. The results of PCA and DFA analyses for e-nose demonstrated that the samples of A. membranaceus, var. mongholicus in different growth years could be distinguished obviously. The contents of chemical composition were similar in same growth years in A. membranaceus, var. mongholicus and different from different growth years. The results of chemical composition analysis indicated that the identification for A. membranaceus, var. mongholicus in different growth years was significant for the quality control. E-nose technique could identify the samples of A. membranaceus, var. mongholicus in different growth years rapidly, sensitively and intactly, and could he applied for the quality control of traditional Chinese medicine.
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