雄黄对肝癌细胞株QGY-7703增殖和凋亡的影响  

Effects of Realgar on the Proliferation and Apoptosis of Human Hepatocel-lular Carcinoma Cell Line QGY-7703

作　　者：杨静[1] 张建军[2] 钟森[3] 

机构地区：[1]雅安市人民医院感染科,四川雅安625000 [2]泸州医学院附属医院感染科,四川泸州646000 [3]成都中医药大学附属医院肝病科,四川成都610072

出　　处：《中外医疗》2015年第2期12-13,共2页China & Foreign Medical Treatment

摘　　要：目的研究中药雄黄主要成分As2S2对人肝癌细胞株QGY-77 03增值和凋亡的影响及其可能机制。方法 2012年9月—2013年4月,分析不同浓度的As2S2处理QGY-7703细胞后,MTT法测定细胞增殖抑制率;流式细胞仪检测细胞凋亡率;免疫细胞化学方法检测PCNA的表达情况。结果较低浓度(7.5 mg/L)的As2S2对QGY-7703细胞的生长无影响;15~120mg/L的As2S2可抑制细胞的生长,具有时间依赖性和浓度依赖性(P<0.01)。As2S2在15~30 mg/L范围内,细胞凋亡率随浓度和作用时间增加而增加(P<0.01);60 mg/L组凋亡率比低浓度组低,表现为继发性坏死细胞增多。As2S2处理组可明显降低PCNA蛋白的阳性表达(P<0.01)。结论 As2S2在一定的浓度范围内可以诱导QGY-7703细胞发生凋亡,通过下调PCNA的表达抑制细胞的增殖,随着作用时间的延长和(或)浓度的提高,As2S2表现出一定的细胞毒作用,促进肿瘤细胞坏死。Objective To study the effect of realgar main component As2S2 on the proliferation and apoptosis of human hepatocel-lular carcinoma cell line QGY-7703 and the possible mechanism. Methods From September 2012 to April 2013 QGY-7703 cell was treated by different concentrations of As2S2 first, then the cell proliferation inhibition rate of QGY-7703 cell was detected by MTT method; the cell apoptosis was tested by flow cytometry; the expression of PCNA was detected by immunocytochemistry method. Results As2S2 at low concentration（7.5mg/L） had no effect on the growth of QGY-7703 cells;As2S2 with the concentration of 15mg/L^120mg/L could inhibit the growth of QGY-7703 cells, the inhibitory rate depended on the concentration and time （P〈0.01）. As2S2 with the concentration of 15 mg/L^30 mg/L, the cell apoptotic rate increased with the increase of concentration and action time （P〈0.01）. The cell apoptotic rate of 60 mg/L group was lower than those of low concentration groups, and the secondary necrotic cells increased. The positive expression level of PCNA protein of As2S2 group decreased significantly（P〈0.01）. Conclusion As2S2 in a certain range of concentration can induce the apoptosis of QGY-7703 cells, inhibit the proliferation of the cells by down-regulation of the expression of PCNA. And as the longer duration of action and （or） the increase of the concentration, As 2S2 showed certain cytotoxic effect, promoting tumor cell necrosis.

关 键 词：雄黄 肝细胞癌 凋亡 

分 类 号：R736[医药卫生—肿瘤]