DC-CIK细胞与顺铂对乳腺癌细胞杀伤作用的研究  

The Expermenal Study of the Cytotoxicity to Breast Cancer Cell Line Using DC-CKI Cells and Cis-platinum

作　　者：底玮 沈方臻[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003

出　　处：《中外医疗》2015年第8期124-126,共3页China & Foreign Medical Treatment

摘　　要：目的研究DC-CIK细胞和不同浓度顺铂对乳腺癌MCF-7细胞的增殖抑制作用。方法将DC细胞和CIK细胞共同培养得到的DC-CIK细胞与顺铂分别作用于MCF-7细胞,共同培养12h、24 h和48 h,MTT法检测MCF-7细胞的增殖抑制情况,对比DC-CIK细胞和顺铂对MCF-7细胞的增殖抑制效果。结果与DC-CIK细胞共培养12 h、24 h与48 h,MCF-7细胞均出现一定的凋亡,增殖受到明显的抑制;与5×10~6个DC-CIK细胞共培养12h与40 ng/mL顺铂对其作用12 h的抑制效果相同,共培养24 h与50 ng/mL顺铂对其作用24 h的效果相同,共培养48 h与60 ng/mL顺铂对其作用24 h的效果相同。结论 该研究为DC-CIK法治疗乳腺癌作出了实验室基础性研究,为临床DC-CIK细胞抑制乳腺癌细胞提供了理论依据。Objective To investigate the in-vitro inhibitory efects of DC（dendritic cel1）-CIK （cytokine-induced killer cel1）cocul-tured cells and cis-platinum against breast cancer cell line MCF-7 cells. Methods In this study, the DC cells and CIK cells co-cultured DC-CIK cells and in MCF-7 cells co-cultured for 12, 24 and 48 hours, using the MTT assay MCF-7 cell proliferation inhibition and using different concentrations the chemotherapy drug cisplatin role in MCF-7 cells, using the same MTT assay MCF-7 cell proliferation inhibition. Then compare the inhibitory effect of DC-CIK cells and the chemotherapy drug cisplatin pro-liferation of MCF-7 cells. Results Addition of DC-CIK cells using MTT assay DC-CIK role in the MCF-7 cell proliferation of MCF-7 before and after the show, regardless of the role of 12, 24 or 48 hours, MCF-7 cells apoptosis, proliferation was significant-ly inhibited. DC-CIK and cisplatin on MCF-7 proliferation inhibition comparison, 5 ×106 cells proliferation of MCF-7 cells 12h inhibition with 40 ng/mL concentration of cisplatin on the proliferation of MCF-7 cells for 12h inhibition equivalent for 24h inhib-ited with 50 ng/mL cisplatin equivalent to the inhibition of proliferation of MCF-7 cells 24h, 48h role in the inhibition of 60 ng /ml of cisplatin on the proliferation of MCF-7 cells the 24h of inhibition basic equivalent. The results show that the DC-CIK cells to inhibit the proliferation of MCF-7 cells. Conclusion This study DC-CIK therapy for breast cancer made a laboratory for basic research. In the clinical treatment of breast cancer has a theoretical and experimental basis.

关 键 词：树突状细胞 细胞因子诱导的杀伤细胞 DC-CIK细胞 乳腺癌 免疫治疗 

分 类 号：R73[医药卫生—肿瘤]