滑膜素对乳腺癌细胞迁移和增殖的影响  

Synoviolin inhibits the migration and proliferation of breast cancer cells

作　　者：徐月梅[1] 帅玄钰 李红妍[1] 黄文斌[2] 苏东明[1,3] 

机构地区：[1]南京医科大学病理学系,江苏南京210029 [2]南京医科大学附属南京医院病理科,江苏南京210012 [3]南京医科大学第二附属医院生物治疗中心,江苏南京210003

出　　处：《南京医科大学学报（自然科学版）》2015年第4期496-502,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：科技部973项目(2011CB504000);国家自然科学基金面上项目(81070656;81171589);江苏省特聘医学专家科研资助专项

摘　　要：目的 :探讨滑膜素(synoviolin)在乳腺癌组织中的表达情况及其对乳腺癌细胞MCF-7增殖和迁移能力的影响。方法 :通过Western blot、免疫组织化学染色及免疫组织芯片技术检测乳腺癌组织中滑膜素的表达水平;在乳腺癌细胞MCF-7中利用腺病毒过表达滑膜素,并以划痕实验、Transwell迁移实验、CCK-8细胞增殖试剂盒等检测其对于乳腺癌细胞MCF-7迁移和增殖能力的影响。结果:滑膜素在乳腺癌组织中的表达水平明显低于相应癌旁组织;过表达滑膜素能抑制乳腺癌细胞MCF-7的增殖和迁移能力;过表达滑膜素能抑制乳腺癌细胞MCF-7发生上皮-间质转化。结论:滑膜素在乳腺癌组织中低表达,可能与抑制乳腺癌细胞的迁移和增殖能力密切相关。Objective：To investigate the synoviolin expression in breast cancer tissues and its effects on the proliferation and migration of breast cancer cell line MCF-7. Methods：The expression of synoviolin in breast cancer tissues was detected by Western blotting and immunohistochemical staining. The proliferation and migration of MCF-7 ceils were detected by CCK-8 kit assay, wound healing assay,and Transwell migration assay. The overexpression of synoviolin in MCF-7 cells was performed by adenoviral vector transfection. Results：The expression of synoviolin in breast cancer tissue was decreased than that of the corresponding peritumoral tissues;Overexpression of synoviolin inhibited the proliferation and migration as well as epithelial-mesenchymal transition （EMT） of the MCF-7 breast cancer cells. Conclusion：Down-regulation of synoviolin in patients with breast cancer may play a critical role in the proliferation and migration of breast cancer.

关 键 词：滑膜素 乳腺癌细胞MCF-7 增殖和迁移 上皮-间质转化 

分 类 号：R737.9[医药卫生—肿瘤]