大鼠髁突软骨在髁突颈骨折和截骨致异常应力下的超微结构改变  

The rat′s cartilage ultrastructure change in abnormal stress by condylar neck fracture and osteotomy

作　　者：陈建中[1] 杨春[1] 刘春雷 朱房勇[3] 胡瑜[1] 李松[1] 

机构地区：[1]昆明医科大学口腔医学院,云南昆明650031 [2]云南文山州医院口腔科,云南文山663000 [3]江苏省无锡市第三人民医院口腔科,江苏无锡214021

出　　处：《重庆医学》2015年第16期2161-2163,共3页Chongqing medicine

基　　金：国家自然科学基金面上项目(30960426;81360163);云南省科技厅-昆明医科大学应用基础联合专项资金项目(2013FZ279)

摘　　要：目的研究髁突在体内受异常应力变化后对髁突软骨超微结构的影响。方法采用4周龄SD大鼠54只建立髁突颈骨折及截骨模型,设截骨组、骨折组及空白对照组,于术后1、3、5周处死,光镜、扫描电镜(SEM)及透射电镜(TEM)观察髁突软骨结构变化。结果光镜显示术后3、5周,截骨组及骨折组髁突总厚度均小于空白对照组。SEM显示截骨组和骨折组术后5周胶原纤维暴露,截骨组手术侧还出现胶原纤维断裂、崩解。TEM显示术后3周截骨组较空白对照组及骨折组肥大软骨细胞成熟分化增强;截骨组术后1周手术侧可见表面纤维排列紊乱甚至缺失。5周截骨组手术侧见胶原纤维排列更为紊乱,纤维层中可见到裂隙。结论体内髁突异常应力变化会持续影响髁突软骨超微结构变化,且跟应力变化的程度相关,进而引起髁突发育的异常。Objective To investigate whether the abnormal stress could cause the ultrastructural change of the rats＇ condylar cartilage in vivo. Methods Fifty-four SD male rats were randomly divided into condylar neck osteotomy operation（OO）group,con- dylar neck fracture operation（FO） group, and control group, with 12 rats in each group. Then the OO group rats, FO group rats and control group rats were executed and sample~ at 1st week, 3rd week and 5th week separately to observe the structure of condyle with light microscope （ LM）, scanning electron microscope （SEM） and transmission electron microscope （TEM）. Results LM showed that the general condylar thickness of OO group and FO group were thinner than that of control group at 3rd and 5th week. SEM showed that the collagen and fibre were exposed in the OO group and FO group at 3rd week and 5th week,the fibre was bro- ken in the OO group at 5th week. TEM showed that the hypertrophic cell in OO group was more mature than those of FO group and control group at 3rd week. The arrange of fibre was disorder in OO group at 1st week,the fibre was more disorder and there was even leak in OO group at 5th week. Conclusion The abnormal stress in vivo could result in the uttrastructural change of the rats＇ condyle,and this change relates with the stess change degree and affects the growth and development of condyle.

关 键 词：髁突 骨折 截骨术 超微结构 

分 类 号：R782.4[医药卫生—口腔医学]