间接免疫荧光法和酶联免疫吸附法检测抗线粒体抗体的分析与比较  

The analysis and comparison of anti-mitochondrial antibody detected by indirect immunofluorescence and enzyme linked immunosorbent assay

作　　者：王艳[1] 刘晓辉[1] 张学智[1] 彭清林[1] 王国春[1] 

机构地区：[1]中日友好医院风湿免疫科,北京100029

出　　处：《中日友好医院学报》2015年第3期154-157,共4页Journal of China-Japan Friendship Hospital

摘　　要：目的：对临床上常用的2种抗线粒体抗体（AMA）的检测方法进行分析比较,探讨抗线粒体抗体M2亚型（AMA-M2）在原发性胆汁性肝硬化（PBC）和其他结缔组织病（CTD）中的抗体滴度特点。方法 ：回顾性分析2011年1月~2013年6月我科采用间接免疫荧光法（IIF）检测AMA和酶联免疫吸附法（ELISA）检测AMA-M2共726例患者的资料。结果 ：ELISA检测抗线粒体抗体阳性率（13.1%）略高于IIF（6.5%）。IIF诊断PBC的敏感度和特异度为78.1%和97.4%,ELISA分别为100%和91.6%。55例AMA-M2阳性而AMA阴性的患者,8例诊断为PBC,35例诊断为其他CTD。PBC组AMA-M2抗体滴度（中位数为64.8RU/ml）略高于其他CTD组（中位数为54.9RU/ml）。结论 ：将IIF和ELISA同时应用于抗线粒体抗体的检测,有助于PBC的临床诊断。Objective：To compare the difference between the results of two methods that was commonly used in clinical testing of antimitochondrial antibody and explore the concentration characteristics of M2 subtype in primary antibody of biliary cirrhosis and the other connective tissue diseases.Methods： Retrospectively analyzed the 726 patients in our hospital with the results of two methods about antantimitochondrial antibody：both in indirect immunofluorescence（IIF）for detecting the total antimitochondrial antibody（AMA）and ELISA for detect- ing antimitochondrial subtype M2 （AMA-M2）from January 2011 to June 2013.Results：The positive rate of ELISA in detection of anti mitochondrial antibody was 13.1% and higher than that of IIF（6.5%）.The sensitivi- ty and specificity for diagnosing primary biliary cirrhosis（PBC）by IIF were 78.4% and 97.4%,while 100% and 91.6% for EL1SA.PBC patients had a higher median concentration of AMA-M2 （64.8RU/ml）than the other CTDs patients （54.gRU/ml）.Conclusion：Combining IIF and ELISA results of AMA testing is helpful for the clinical diagnosis of PBC.

关 键 词：抗线粒体抗体 抗线粒体抗体M2亚型 原发性胆汁性肝硬化 结缔组织病 

分 类 号：R575.2[医药卫生—消化系统] R593.2[医药卫生—内科学]