基于气相色谱-质谱技术对用咪康唑处理的白念珠菌的代谢组学研究  

Metabolic analysis of miconazole-treated Candida albicans by GC-MS

作　　者：杨宇[1] 王慧[1] 曹颖瑛[2] 朱臻宇[1] 

机构地区：[1]第二军医大学药学院药物分析学教研室,上海200433 [2]第二军医大学药学院新药研究中心,上海200433

出　　处：《药学实践杂志》2015年第3期209-212,共4页Journal of Pharmaceutical Practice

摘　　要：目的基于代谢组学方法研究咪康唑作用前后白念珠菌的代谢谱差异,寻找潜在的生物标志物,探讨药物作用机制。方法采用气相色谱-质谱方法测定咪康唑给药前后白念珠菌的代谢特征谱,利用多元统计分析方法进行比较,筛选潜在的生物标志物,并探讨代谢物产生差异的原因。结果经分析,筛选出23个潜在的生物标志物,主要参与了氨基酸代谢、三羧酸循环、氧化应激、糖酵解和磷脂代谢等代谢路径。结论咪康唑通过影响多种代谢路径而发挥抗真菌活性。Objective To investigate the metabolism in miconazole-treated Candida albicans ,search for possible biomar-kers and discuss the mechanism of miconazole .Methods GC-MS was employed to determine the metabolic fingerprint of Can-dida albicans before and after treatment with miconazole ,and the difference based on multivariate was compared by statistical analysis ,the potential biomarkers were screened out and the mechanism of miconazole was discussed .Results Twenty three metabolites was screened out as potential biomarkers ,and they were primarily involved in amino acid metabolism ,citrate cycle , glycolysis and lipid metabolism .Conclusion The antifungal activity of miconazole was played by affecting a variety of metabolic pathways .

关 键 词：代谢组学 气相色谱-质谱 白念珠菌 咪康唑 

分 类 号：R379.4[医药卫生—病原生物学]