缺血预适应对脑缺血再灌注大鼠皮质区血管内皮生长因子及存活素表达的影响  

Effect of ischemia preconditioning on expression of VEGF and survivin in rat cortical area following cerebral I/R

作　　者：张会玲[1] 李世英[1] 李峥[2] 张晋霞[1] 贺永贵[1] 余红[3] 刘斌[1] 

机构地区：[1]河北联合大学附属医院神经内一科,唐山063000 [2]唐山玉田县医院神经内科 [3]河北联合大学基础医学院药理教研室

出　　处：《中华老年心脑血管病杂志》2015年第6期645-648,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：河北省2013年医学科学研究重点课题计划(20130382)

摘　　要：目的观察缺血预适应对局灶性脑缺血再灌注大鼠梗死灶周边皮质区血管内皮生长因子(VEGF)、存活素表达的影响,探讨其脑保护机制。方法 SD大鼠130只,随机分为假手术组10只、脑缺血组60只及预适应组60只,脑缺血组和预适应组按缺血后再灌注时间不同分为再灌注2、6、12、24、48和72h时间点,每个时间点10只。采用线栓法闭塞右侧大脑中动脉,制备大鼠局灶性脑缺血再灌注预适应模型。应用免疫组织化学法及Western blot检测观察梗死灶周边皮质区VEGF及存活素的表达。结果假手术组VEGF阳性细胞、存活素阳性细胞及皮质区VEGF蛋白和存活素蛋白表达较少。与脑缺血组比较,预适应组2、6、12、24、48和72hVEGF及存活素阳性细胞和蛋白表达明显升高(P<0.05,P<0.01)。结论脑缺血预适应可诱导VEGF和存活素表达上调,这可能是脑缺血预适应诱导脑缺血耐受的脑保护作用机制之一。Objective To study the mechanism of ischemia preconditioning for protection of brain by observing its effect on expression of VEGF and survivin in rat cortical area following focal cerebral I/R.Methods One hundred and thirty SD rats were randomly divided into sham operation（SO）group（n=10）,middle cerebral artery occlusion（MCAO）group（n=60）and cerebral ischemia preconditioning（CIP）group（n=60）.Rats in MCAO group and CIP group were further divided into 2hreperfusion group,6hreperfusion group,12 hreperfusion group,24 hreperfusion group,48 hreperfusion group and 72 hreperfusion group（10in each group）.A rat focal CIP model was established by Longa occlusion of the right middle cerebral artery.Expression of VEGF and survivin in cortical area was detected by Western blot with immunohistochemical staining.Results The expression levels of VEGF and survivin were significantly higher in CIP group than in MCAO group at 2,6,12,24,48 and 72hafter I/R（P〈0.05,P〈0.01）.Conclusion CIP can upregulate the expression of VEGF and survivin,which is one of the mechanisms underlying CIP-induced ischemia tolerance.

关 键 词：脑缺血 再灌注 血管内皮生长因子类 细胞增殖 

分 类 号：R743.3[医药卫生—神经病学与精神病学]