米根霉交替呼吸途径高活性突变株的选育及其对富马酸合成的影响  

Screening of Rhizopus oryzae mutants with higher activity of alternative respiration for fumaric acid production

作　　者：孙小龙[1] 付永前[1] 徐晴[1] 李霜[1] 黄和[1] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,江苏南京210009

出　　处：《生物加工过程》2015年第3期26-30,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2013CB733605);国家高技术研究发展计划(863计划)重点项目(2011AA02A206);国家自然科学基金(21076104;21106065;21106091)

摘　　要：米根霉交替呼吸强度与富马酸合成之间存在重要关联。以米根霉F-14为出发菌株,通过常压室温等离子体(ARTP)离子诱变技术,筛选出1株交替呼吸强度增强的突变菌株S-1。该菌株的交替呼吸初始强度是出发菌株F-14的3.5倍,然而,该菌株富马酸积累量(28 g/L)却远低于出发菌株(42 g/L)。进一步考察突变株S-1和出发株F-14菌株在发酵过程中交替呼吸及富马酸生产强度,总呼吸变化和还原力NADH/NAD+的变化。结果表明:突变株在富马酸发酵初始阶段,过高的交替呼吸强度反而降低了富马酸的生产速率,并导致了发酵后期菌体的早衰现象。交替呼吸强度与发酵进程存在适配性,只有当适配性达到最佳状态时,才有利于产物的高效积累。During fumaric acid fermentation, alternative respiration is associated with fumaric acid productivity in Rhizopus oryzae-S-1 mutant with higher activity of alternative respiration was screened by atmospheric and room temperature plasma （ ARTP ） ion mutagenesis using Rhizopus oryzae F-14 as the original strain. The activity of alternative respiration in S-1mutant is 3.5-fold than that of F-14. However, fumaric acid production （ 28 g/L ） was much lower than that of the original strain （ 42 g/L ） . The relationship between alternative respiration ratio and fumaric acid productivity, the change of total respiration activity and NADH/NAD^＋ of S-1 and F-14 in the fermentation process were further studied. At the initial fermentation stage higher activity of alternative respiration in S-1 mutant reduced the fumaric acid productivity,and ultimately led to the premature aging phenomenon in the fermentation. It means that the activity of alternative respiration should adapt the fumaric acid productivity.

关 键 词：米根霉 富马酸 交替呼吸 生产强度 

分 类 号：TK6[动力工程及工程热物理—生物能] Q819[生物学—生物工程]