ERK信号通路在人牙髓干细胞增殖与分化中的作用  

The effect of ERK signaling pathway on the proliferation and differentiation of human dental pulp stem cells

作　　者：马逢乐 刘宝刚 王娟[1] 何欣遥 王志华 何文喜 

机构地区：[1]军事口腔医学国家重点实验室陕西省口腔医学重点实验室第四军医大学口腔医院牙体牙髓病科,陕西西安710032 [2]第二炮兵总医院礼士路门诊部,北京100820

出　　处：《牙体牙髓牙周病学杂志》2015年第5期277-281,262,共6页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助(81271125;81470733)

摘　　要：目的:探讨细胞外信号调节激酶(ERK)通路是否参与对人牙髓干细胞(h DPSCs)增殖及分化过程的调控。方法:用不同浓度的ERK通路抑制剂U0126干预h DPSCs,用CCK-8法检测细胞增殖;在矿化液诱导条件下,用不同浓度U0126干预h DPSCs 14 d,茜素红染色观察矿化结节的形成,RT-PCR检测成骨/成牙相关基因ALP、OCN、DSPP及BSP的表达;Western blot检测U0126(25μmol/L)干预后,ERK通路活性的变化。结果:不同浓度的U0126对h DPSCs的增殖能力无显著影响(P>0.05);与对照组相比,不同浓度的U0126对矿化结节的形成和成骨/成牙相关基因ALP、OCN、DSPP及BSP的表达均有抑制作用,25μmol/L U0126的抑制作用最明显(P<0.05);Western blot结果显示随着时间的延长,p-ERK的表达量逐步增加,在加入25μmol/L U0126后,p-ERK表达量下降(P<0.05)。结论:ERK信号通路可能不参与对h DPSCs增殖的调控,而在促进h DPSCs成骨/成牙分化过程中起调控作用。AIM： To investigate the effects of tamoxifen on the osteoblastic/odontoblastic differentiation of human dental pulp stem cells（ h DPSCs） in vitro. METHODS： h DPSCs were treated by different concentrations of tamoxifen. Kit-8 assay was applied to assess cell proliferation. After mineralization induction culture for 2 weeks,alizarin red S staining was used to observe the mineral nodules,the mRNA expression of the alkaline phosphatase（ ALP）,osteonectin（ OCN）,bone sialoprotein（ BSP） and dentin sialophosphoprotein（ DSPP） was detected by RT-PCR.RESULITS： Tamoxifen at 10μmol / L inhibited h DPSCs proliferation and lower concentration showed no effect. All concentration of tamoxifen increased mineral nodule formation of h DPSCs,5 μmol / L tamoxifen showed the most significant effect（ P〈0. 05）. Furthermore,the mRNA expression of ALP,OCN,BSP and DSPP was markedly upregulated by 5 μmol / L tamoxifen treatment. CONCLUSION： Tamoxifen promotes mineralization and mineralization- related genes expression and might play a vital role on the differentiation of h DPSCs.

关 键 词：人牙髓干细胞(hDPSCs) ERK信号通路 增殖 分化 

分 类 号：R780.2[医药卫生—口腔医学]