大鼠实验性牙髓炎痛敏模型的建立及检验  

Establishment and verification of rat experimental hyperalgesic pulpitis

作　　者：高磊 栗红师 闫磊 孙亚男 张海[3] 吴礼安 

机构地区：[1]军事口腔医学国家重点实验室陕西省口腔医学重点实验室第四军医大学口腔医院儿童口腔科,陕西西安710032 [2]解放军空军总医院,北京100036 [3]第四军医大学实验动物中心,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2015年第5期299-303,328,共6页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金面上项目(81170929);陕西省自然科学基金面上项目(2014JM4114)

摘　　要：目的:验证牙髓暴露法建立大鼠实验性牙髓炎痛敏模型的可行性。方法:取健康雄性SD大鼠36只,随机分为2组;对照组(n=10)麻醉后不作任何处理,牙髓炎模型组(n=26)麻醉后于左侧上颌第一磨牙穿髓并使之暴露于口腔。然后,每组中各随机抽取6只分别检测其术前及术后1、3、7、14 d的擦面行为。对照组所余4只于术后2 h取延髓及牙髓,牙髓炎组所余20只分别于术后2 h取延髓,1、3、7、14 d取牙髓;用免疫荧光染色检测延髓中c-Fos蛋白及牙髓中TNF-α的表达,HE染色观察牙髓炎症情况。结果:1牙髓炎组在建模术后1 d时的擦面总时间最高,其次为术后3 d组,两者分别与组内其他各时间相比,以及与同一时间点的对照组相比差异均有统计学意义(P<0.05);2HE染色显示,牙髓暴露1 d时即可见明显炎症,术后1、3、7、14 d,其坏死组织的边缘分别位于髓角和根上、中、下1/3处;3牙髓炎组在术后1、3、7、14 d各时间点的TNF-α表达强度的平均光密度值均明显高于对照组(P<0.05);4术后2 h牙髓炎组和对照组延髓背角的c-Fos阳性细胞数为176.8±14.82和18.2±4.08(P<0.05)。结论:牙髓暴露法能有效模拟临床牙髓炎及其痛敏过程。AIM： To establish and verify rat experimental hyperalgesic pulpitis. METHODS： 36 male SD rats were randomly divided into 2 groups. The rats in control group（ n = 10） received no treatment after anesthesia,while the left maxillary first molar pulp of the rats in the pulpitis group（ n = 26） were exposed after anesthesia. 6 rats of each group were randomly selected for face grooming test before and after 1,3,7 and 14 d of surgery respectively. The medulla oblongatas and pulps of the remaining 4 rats in the control group were taken after 2 h of surgery. The remaining 20 rats in the pulpitis group were randomly divided into 5 groups（ n = 4）,the pulps were taken after 1,3,7 and 14 d of surgery respectively. HE staining,TNF-α and c-Fos immunofluorescence staining were conducted to measure the changes of pulp and bulbus medullae. RESULTS： The face Grooming after 1 d or 3 d of surgery in pulpitis groups were longer than that in the control group（ P 0. 05）. In the pulpitis groups longest face grooming was observed 1d followed by 3 d（ P 0. 05） after surgery. HE staining revealed that 1,3,7 and 14 d after surgery,inflammation and necrotic tissues appeared at the pulp horn,cervical-third,middle-third and apical-third of the root respectively. The mean optical density of TNF-α at various time points of pulpitis group were significantly higher than that of control group（ P 0. 05）. In pulpitis group highest TNF-α level was observed 1d followed by 3 and 7 d after surgery（ P 0. 05）,the lowest at 14 d after surgery（ P 0. 05）. c-Fos immunostaining results showed that c-Fos-positive cells were significantly increased in medullary dorsal horn after 2 h of pulp exposure. CONCLUON： Pulp exposure method can effectively establish rat experimental hyperalgesic pulpitis madel,which simulates the process of clinical pulpitis and hyperalgesia.

关 键 词：开髓 实验性牙髓炎 痛行为 免疫荧光染色 C-FOS 

分 类 号：R780.2[医药卫生—口腔医学]