超声辐照联合双配体载药纳米粒对人乳腺癌耐紫杉醇细胞株细胞毒性的效果评价  

The cytotoxity of paclitaxel-nanoparticles with dual ligands combined with ultrasound irradiation on drug- resistant breast cancer cells

作　　者：洪少馥[1] 王冬晓[1] 杨莉[2] 罗婉贤 王莺[3] 李颖嘉[1] 

机构地区：[1]南方医科大学南方医院超声科,广州510515 [2]南方医科大学南方医院药剂科 [3]南方医科大学南方医院肿瘤研究所

出　　处：《中华超声影像学杂志》2015年第5期435-439,共5页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金面上项目（81371559）;广东省科技计划项目（20118010400018）;广东省科技计划项目（20138010404024）

摘　　要：目的对人乳腺癌紫杉醇敏感细胞MCF-7及紫杉醇耐药细胞MCF-7／Tax的超声辐照条件进行筛选，在优化条件下研究超声辐照联合双配体载紫杉醇纳米粒逆转肿瘤多药耐药效果。方法设置不同声强、辐照时间、微泡／培基体积比，超声辐照后台盼蓝拒染法测算细胞存活率、荧光显微镜观察纳米粒子在两种细胞的荧光强度，筛选出对两种细胞生长活性无明显抑制的最优超声辐照条件。根据以上筛选条件，开展MTT实验观察超声辐照联合双配体纳米粒对两种细胞毒性效果。结果声强0．6W／cm^2、辐照时间80s、微泡原液与培基体积比1：10为MCF-7细胞的最优辐照条件；声强0．6w／cm^2、辐照时间80s、微泡原液与培基体积比1：20为MCF-7／Tax细胞的最优辐照条件。上述辐照条件下超声辐照联合双配体载紫杉醇纳米粒作用组对乳腺癌MCF-7细胞和MCF-7／Tax细胞显示出较好的杀伤作用（IC50-MCP-7 1.43ng／ml，IC50-MCP-7／Tax〈800ng／ml），且效果明显优于单独双配体载药纳米粒作用组（P〈0.01，P〈0．05）。结论超声辐照在适宜条件下可增强双配体载药纳米粒对耐药细胞的敏感性，表明该策略对逆转肿瘤多药耐药具有应用价值。Objective To screening optimized ultrasound （US） condition for MCF-7 cells and MCF- 7/Tax cells, and to investigate the effect of dual-ligands nanoparticles （NPs） combined with US on overcoming multidrug resistance （MDR）. Methods To obtain optimized US condition, cell viability and cellular uptake of NPs treated with MCF-7 cells and MCF-7/Tax cells were detected under different ultrasonic parameters including ultrasound intensity, exposure duration and microbubble concentration. MTT assay was performed to observe the effect of NPs combined with US on cells. Results The optimal US conditions for MCF-7 cells was irradiation intensity 0.6 W/cm2, irradiation time 80 s, and the MBs concentration with volume ratio of microbubbles to medium was 1 ： 10;and for MCF-7/Tax cells,irradiation intensity 0.6 W/cm2 ,irradiation time 80 s,and the MBs concentration with volume ratio of microbubbles to medium was 1 ： 20. Compared with individual NPs,NPs assisted by US exhibited greater killing ability for MCF-7 （IC50 l.43 ng/ml） and MCF-7/Tax ceils （IC50〈800 ng/ml）. Conclusions US effect could enhance drug sensitivity of the dual-functionized NPs for MCF-7/Tax cells, indicating that US irradiation had potential assisted modality to reverse tumor MDR.

关 键 词：超声检查 微气泡 多药耐药 双配体载药纳米粒 乳腺肿瘤 

分 类 号：R730.5[医药卫生—肿瘤] R454.3[医药卫生—临床医学]