前列腺癌新生血管生成与ROS和PI3K/AKT信号通路相关蛋白的关系  

Relationship between ROS,PI3K/AKT signaling pathways associated protein and prostate cancer angiogenesis

作　　者：郭忠英[1] 傅广波[2] 刘海燕[1] 孙苏安[1] 

机构地区：[1]南京医科大学附属淮安第一医院病理科,淮安223300 [2]南京医科大学附属淮安第一医院泌尿外科,淮安223300

出　　处：《临床与实验病理学杂志》2015年第5期552-555,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：淮安市科技计划(HA2012040)

摘　　要：目的检测前列腺癌(prostatic cancer,PCa)和癌旁组织中活性氧(reactive oxygen species,ROS)的表达、PI3K/AKT信号通路中相关基因蛋白p70S6K1、VEGF的表达及微血管密度(micro-vascular density,MVD)值,分析ROS与p70S6K1、VEGF蛋白之间的相关性,以及ROS、p70S6K1、VEGF蛋白表达与PCa血管生成的关系。方法采用免疫荧光法检测PCa和癌旁组织内的ROS水平;免疫组化SP法检测PI3K/AKT通路相关基因蛋白p70S6K1、VEGF表达及MVD计数情况。结果 PCa组织中ROS的表达水平明显高于癌旁组织,差异有统计学意义(P<0.05);PCa组织中p70S6K1、VEGF蛋白表达及MVD值高于癌旁组织,差异有统计学意义(P<0.05),PCa组织中ROS、p70S6K1、VEGF三者表达间均呈正相关,且ROS、p70S6K1及VEGF表达均与MVD值呈正相关。结论 (1)PCa组织中ROS表达水平明显高于癌旁组织。(2)PCa组织中PI3K/AKT通路相关基因蛋白p70S6K1、VEGF蛋白表达高于癌旁组织,PCa中MVD值高于癌旁组织,提示PI3K/AKT信号通路在肿瘤血管生成中起一定作用。(3)PCa组织中ROS表达水平和PI3K/AKT通路相关基因蛋白p70S6K1、VEGF表达及MVD值均呈正相关,提示ROS可能通过PI3K/AKT信号通路,对PCa发生、发展起正调控作用。Purpose To investigate the expression of reactive oxygen species （ROS） and PI3K/AKT signaling pathway associated gene protein p70S6K1, VEGF in prostate cancer and adjacent tissues, and to determine their relationship with micro-vascular density （ MVD） , as well as the relationship between themselves. Methods The expression of ROS was detected by immunofluorescence, and the expression of p70S6K1, VEGF and MVD counting were detected by immunohistochemistry. Results Compared to adjacent tis-sues, the expression of ROS, p70S6K1, VEGF and MVD in PCa were increased considerablely （P〈0. 05）. In prostate cancer the ex-pression of ROS were positively correlated to the expression of p70S6K1, VEGF protein, and the expression of ROS and p70S6K1, VEGF were positively correlated to the number of MVD. Conclusion （1）The expression level of ROS in prostate cancer tissues was significantly higher than adjacent tissues. （2） The expression of PI3K/AKT pathway associated gene protein p70S6K1, VEGF protein and MVD counting in prostate cancer tissue was higher than the adjacent tissues, suggesting PI3K/AKT signaling pathways may play a role in tumor angiogenesis. （3） The expression of ROS and PI3K/AKT pathway associated gene protein p70S6K1, VEGF and MVD counting in prostate cancer tissues was positively correlated, suggesting that ROS may play a positive regulatory role for the development of prostate cancer by PI3K/AKT signaling pathway.

关 键 词：前列腺肿瘤 活性氧 p70S6K1 肿瘤新生血管生成 

分 类 号：R737.25[医药卫生—肿瘤]