Nd:YAG激光对鳞状细胞癌CAL-27细胞增殖的影响  

The effect of Nd: YAG laser irradiation on squamous carcinoma CAL-27 cell proliferation

作　　者：马浩然[1,2] 吴国英[1,2] 李留炀[1,2] 尚进 钱棱[1,2] 陶楠楠 钱梦柯[1,2] 王培 仇嘉颖[1,2] 

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室 [2]南京医科大学附属口腔医院牙体牙髓黏膜科,江苏南京210029

出　　处：《口腔医学》2015年第5期362-364,共3页Stomatology

基　　金：江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的研究Nd:YAG激光对鳞状细胞癌(鳞癌)CAL-27细胞的生物学效应。方法用脉冲1064 nm Nd:YAG激光连续照射体外培养处于对数生长期的鳞癌CAL-27细胞,分组照射后倒置显微镜下观察细胞形态,培养1、3、5 d后利用MTT法测定OD值,以此来研究激光对鳞癌CAL-27细胞增殖的影响。结果低剂量激光照射时,CAL-27细胞的OD值随着激光照射剂量的增加而升高(P<0.05);而高剂量激光照射时,CAL-27细胞的OD值随着激光照射剂量的增加而降低(P<0.05)。结论 1 064 nm Nd:YAG激光低剂量照射鳞癌CAL-27细胞,细胞表现形态规则、密度增大,增殖活跃;而高剂量照射鳞癌CAL-27细胞后,细胞形态不规则、密度降低,增殖受到抑制,对指导临床应用有一定参考价值。Objective To investigate the biological effect of Nd：YAG laser irradiation on the squamous carcinoma CAL-27 cell pro- liferation. Methods Squamous carcinoma CAL-27 cells were developed in the vitro,and when they were in the logarithmic phase,the cells were irradiated with different doses of Nd ：YAG laser and observed under inverted microscopes. Then,the OD value was measured after 1,3, and 5 days. Results A significant stimulative and inhibitive effect was found respectively of the low and high intensity 1 064 nm laser （P 〈 0.05） on squamous carcinoma CAL-27 eel1 proliferation. Conclusions By low intensity 1064 nm Nd：YAG laser, the squamous carcinoma CAL-27 cell proliferation can be stimulated,the cells are morphologically ruled and their density increases. In con- trast,the proliferation can be inhibited by high intensity laser,the ceils are irregularly shaped and their density decreases. This finding will have a good reference value for the clinical application.

关 键 词：ND YAG激光 鳞状细胞癌 增殖 

分 类 号：R780.2[医药卫生—口腔医学]