脂多糖和植物血凝素对小鼠外周血单个核细胞microRNA水平的影响  

Effects of lipopolysaccharide and phytohaemagglutinin on the level of microRNAs in mouse peripheral blood mononuclear cells

作　　者：朱波[1] 王文红[2] 许秋桂 王瑞[1] 熊御云[1] 严佳婧 舒扬[1] 焦志军[1] 

机构地区：[1]江苏大学附属医院检验科,江苏镇江212001 [2]江苏大学医学院病原生物学系,江苏镇江212001

出　　处：《细胞与分子免疫学杂志》2015年第7期865-868,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81172871;81373217);江苏省科技厅科技创新与成果转化(生命健康科技)专项资金(BL2012057);江苏大学高级人才启动基金(14JDG071)

摘　　要：目的研究脂多糖(LPS)和植物血凝素(PHA)两种淋巴细胞多克隆激活剂对小鼠外周血单个核细胞(PBMC)中microRNA(miRNA)水平的影响。方法分别采用100 ng/m L LPS刺激培养1、2 h的小鼠PBMC,2.5μg/m L PHA刺激培养2、4 h的小鼠PBMC。应用实时荧光定量PCR检测培养细胞和上清中miR-9、miR-122-3p、miR-181d及miR-342-3p的表达水平。结果与相应空白对照组相比,LPS刺激1 h或2 h后,培养上清及PBMC中miR-9、miR-122-3p、miR-181d及miR-342-3p的表达水平未见明显改变。PHA刺激未改变培养上清和PBMC中miR-9、miR-122-3p及miR-342-3p的水平,但显著上调培养上清和PBMC中miR-181d的水平。PHA刺激4 h组与PHA刺激2 h组间,miR-181d表达水平未见明显差异。结论 PHA刺激可促进小鼠PBMC中miR-181d的表达与分泌。Objective To investigate the effects of lipopolysaccharide （LPS） and phytohaemagglutinin （PHA） on the level of microRNAs in mouse peripheral blood mononuclear cells （PBMCs）. Methods The mouse PBMCs were cultured in vitro and stimulated with 100 ng/mL LPS for 1 hour or 2 hours and 2.5 μg/mL phytohaemagglutinin （PHA） for 2 hours or 4 hours, respectively. The levels of miR-9, miR-122-3p, miR-]81d and miR-342-3p in both PBMCs and culture supernatants were determined by real-time quantitative PCR （qRT-PCR）. Results The levels of miR-9, miR-122-3p, mJR-181d and miR-342-3p in PBMCs and culture supernatants had no significant difference between blank control and LPS stimulation group, nor did between LPS stimulation 2 hours group and t hour group. The levels of miR-9, miR-]22-3p and miR-342-3p in PBMCs and culture supernatant had no significant difference between blank control and PHA stimulation group, nor did between PHA stimulation 4 hours group and 2 hours group. However, compared with blank control, the expression of miR-t8]d significantly increased after PHA stimulation, while the time of stimulation did not affect the miR-181d level significantly. Conclusion The stimulation of PHA could promote the expression and secretion of miR-181d in mouse PBMCs.

关 键 词：脂多糖 植物血凝素 外周血单个核细胞 血浆microRNA 

分 类 号：R735.7[医药卫生—肿瘤] R331.144[医药卫生—临床医学]