肿瘤坏死因子α增强全反式维甲酸诱导早幼粒白血病细胞的凋亡  

Tumor necrosis factor alpha enhances apoptosis of all-trans retinoic acid-induced promyelocytic leukemia cells

作　　者：李萌璐 杨欢[1] 王长莹 姚智[1] 高颖[1] 

机构地区：[1]天津医科大学基础医学院免疫学系天津市细胞与分子免疫重点实验室国家教育部免疫微环境与疾病重点实验室,天津300070

出　　处：《细胞与分子免疫学杂志》2015年第7期869-872,878,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(31071217)

摘　　要：目的研究肿瘤坏死因子α(TNF-α)对全反式维甲酸(ATRA)诱导人早幼粒白血病细胞凋亡的影响。方法分别单独使用ATRA或者联合使用ATRA与TNF-α作用于NB4人急性早幼粒白血病细胞,细胞计数检测细胞增殖,annexin V-FITC/PI染色检测细胞凋亡,流式细胞术检测细胞表面CD11b表达的变化。结果与单独使用ATRA相比,联合使用ATRA和TNF-α时,NB4细胞在整个分化过程中增殖速度更慢、凋亡率更高,在分化第2天CD11b阳性细胞的比例更高。结论 TNF-α可能具有增强ATRA诱导早幼粒白血病细胞分化及凋亡的作用。Objective To study the effect of tumor necrosis factor alpha （TNF-α） on apoptosis of human promyelocytic leukemia cells induced by all-trans retinoic acid （ATRA）. Methods Human acute promyelocytic leukemia NB4 cells were treated either by ATRA or by ATRA plus TNF-α. Cell proliferation and apoptosis were detected by cell counting and annexin V-FITC/PI staining, respectively. Changes of CDllb expression on NB4 cells were observed by flow cytometry. Results Compared with NB4 cells treated with ATRA alone, NB4 cells treated with ATRA plus TNF-a showed slower proliferation and a higher rate of apoptosis during the whole process of differentiation, and had a higher ratio of CDIIb positive cells on the second day of differentiation. Conclusion TNF-α may have potential for strengthening the differentiation and apoptosis of acute promyelocytic leukemia cells induced by ATRA.

关 键 词：急性早幼粒细胞白血病 肿瘤坏死因子Α 全反式维甲酸 细胞凋亡 细胞分化 

分 类 号：R733.71[医药卫生—肿瘤] Q279[医药卫生—临床医学]