TNFSF14高表达的Tca8113舌癌细胞增殖及迁移能力增强  

The capacity of proliferation and migration is higher in Tca8113 cells with up-regulated TNFSF14 expression

作　　者：高维岳[1] 王家奎[1] 程文晓[1] 秦子顺[1] 殷丽华[1] 余占海[1] 

机构地区：[1]兰州大学口腔医学院,甘肃兰州730000

出　　处：《细胞与分子免疫学杂志》2015年第7期923-927,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金青年基金项目(81102712);中央高校基本科研业务费专项资金项目(861500)

摘　　要：目的构建肿瘤坏死因子超家族成员14(TNFSF14)基因慢病毒载体,体外感染人舌癌Tca8113细胞,观察TNFSF14基因对Tca8113细胞的影响。方法将TNFSF14基因进行慢病毒包装,感染舌癌Tca8113细胞,通过嘌呤霉素筛选后,实时定量PCR法检测TNFSF14在Tca8113中的表达,MTT检测感染TNFSF14基因后Tca8113细胞的增殖活性,流式细胞术分析细胞周期变化,TranswellTM迁移实验检测细胞迁移能力的变化。结果慢病毒感染后,检测到细胞中TNFSF14 mRNA较对照组表达量升高,MTT法和流式细胞术结果显示TNFSF14可促进Tca8113细胞增殖,TranswellTM实验显示TNFSF14促进Tca8113细胞迁移。结论高表达TNFSF14的Tca8113细胞增殖及迁移能力增强。Objective To construct a lentiviral vector carrying tumor necrosis factor superfamily member 14 （TNFSF14） gene, infect tongue cancer Tca8113 cells in vitro, and observe the effect on infected Tca8113 cells. Methods A lentiviral vector containing TNFSF14 gene was constructed and used to infect the Tca81 13 cells. After selected by puromycin, the level of TNFSF14 mRNA in TcaSl13 cells was detected by real-time quantitative PCR. Cell proliferation activity and cell circle were determined respectively by M＇rT assay and flow cytometry （FCM）. And the cell migration ability was measured by TranswellTM assay. Results Compared with the control group, the expression of TNFSF14 mRNA increased in the infected cells. Ml-r assay and FCM showed TNFSF14 promoted the proliferation of TcaS113 cells. Transwell^TM assay showed TNFSF14 boosted the migration ability of Tca8113 cells. Conclusion The proliferation and migration would be enhanced in Tca8113 cells with over-expressed TNFSF14.

关 键 词：肿瘤坏死因子超家族成员14 慢病毒载体 TCA8113 

分 类 号：R739.86[医药卫生—肿瘤] Q279[医药卫生—临床医学]