CD147介导CD4^+肿瘤浸润淋巴细胞促进乳腺癌进展  

Highly expressed CD147 on CD4^+ tumor infiltrating lymphocytes promotes the progress of breast cancer

作　　者：争鸣 张欣[2] 郭慧芳[1,2] 樊春梅[2] 陈志南[1] 朱平[2] 

机构地区：[1]第四军医大学细胞生物学教研室,细胞工程研究中心,陕西西安710032 [2]第四军医大学西京医院临床免疫科,全军风湿免疫专科研究所,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2015年第7期961-964,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金重点项目(81030058)

摘　　要：目的分析各T淋巴细胞亚群中CD147的表达水平以及CD147表达量在健康对照者外周血,乳腺癌患者外周血、淋巴结和肿瘤浸润淋巴细胞中CD4+T细胞的变化,分析CD147对乳腺癌患者预后的影响。方法采用Robust Multi-array Averaging的标准化方法提取GEO芯片GSE14308、GSE36765和GSE6532中的CD147表达数据。不同的基因表达水平的比较采用方差分析,生存曲线采用Log-Rank检验以及Cox比例风险回归模型进行多因素分析。结果 CD147在C57BL/6J小鼠淋巴结中的Th1细胞、Th2细胞、Th17细胞表达水平是Th0细胞的8~12倍。在乳腺癌患者的CD4+肿瘤浸润淋巴细胞中,CD147的表达水平显著高于健康对照者外周血、乳腺癌患者外周血和淋巴结中的CD4+T细胞。Log-Rank分析中,CD147表达量高低与无复发生存时间显著相关。Cox多元回归模型显示CD147高表达是乳腺癌复发的危险因素(风险度=1.764;95%可信区间:1.088~2.859)。结论 CD147可能通过上调CD4+T细胞的功能参与炎症反应,促进乳腺癌的进展。Objective To investigate the expression of CD147 in CD4＋ tumor infiltrating lymphocytes and explore the clinical significance of CD147 transcripts in breast cancer patients by comparing CD147 level in healthy controls＇ peripheral blood, breast cancer patients＇ peripheral blood, lymph nodes and CD4^＋ tumor infiltrating lymphocytes. Methods CD147 expression data were derived by Robust Multi-array Averaging method from three different microarrays, GSE14308, GSE36765 and GSE6532. ANOVA, Kaplan-Meier curve and multivariate Cox proportional hazards model were used for the statistical analysis. Results The expression of CD147 in Thl, Th2 and Thl7 cells was 8 - 12 times higher than that in ThO cells in normal C57BL/6J mice. The expression level of CD147 in the CD4^＋ tumor infiltrating lymphocytes from breast cancer patients was significantly higher than that in the CD4^＋ lymphocytes from peripheral blood of healthy donors, peripheral blood and axillary lymph nodes of breast cancer patients. High expression of CD147 was significantly associated with an increased risk of relapse in the Kaplan-Meier curve analysis （ Log-Rank, P = 0.019） and multivariate analysis （ hazard ratio = 1. 764; 95% confidence interval： 1. 088 - 2. 859 ）. Conclusion CD147 might promote the progress of breast cancer through up-regulating CD4^＋ tumor infiltrating lymphocytes and aggravating inflammatory reaction.

关 键 词：CDL47 TM细胞 TH2细胞 THL7细胞 肿瘤浸润淋巴细胞 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤] R392.11[医药卫生—临床医学]