脐带间充质干细胞移植治疗脊髓损伤:不同方式的疗效对比  

Transplantation of human umbilical cord derived-mesenchymal stem cells by different ways for the treatment of spinal cord injury

作　　者：刘雨亮[1] 李军[1] 何玉芹[1,2] 卓锋[1] 魏开斌[1] 

机构地区：[1]泰安市中心医院,山东省泰安市271000 [2]单县中医院,山东省菏泽市274300

出　　处：《中国组织工程研究》2015年第14期2192-2198,共7页Chinese Journal of Tissue Engineering Research

基　　金：泰安市科技发展计划(20113076);项目名称:脐带间充质干细胞不同移植方式对脊髓损伤疗效观察~~

摘　　要：背景:目前,来源于人脐带的间充质干细胞移植途径主要为局部移植,存在创伤大、出血多、并发症多等缺点,限制了其在临床上的广泛应用。目的:探讨静脉移植人脐带间充质干细胞治疗脊髓损伤的可行性。方法:80只Wistar大鼠成功制备脊髓打击模型,随机分为5组:空白对照组10只大鼠不进行任何移植;DMEM局部移植组15只、DMEM静脉移植组15只大鼠分别通过局部、静脉途径移植DMEM;细胞局部移植组20只、细胞静脉移植组20只大鼠分别通过局部、静脉途径移植人脐带间充质干细胞悬液。术后定期行BBB评分、组织学观察和免疫组化检测来评价脊髓修复情况。结果与结论:移植后1 d-2周各组间BBB评分差距小,差异无显著性意义;移植后4-12周,细胞局部移植组、细胞静脉移植组BBB评分明显高于其他3组,差异有显著性意义;细胞局部移植组、细胞静脉移植组间差异无显著性意义(P>0.05)。组织学观察显示,细胞局部移植组、细胞静脉移植组损伤局部形成的空洞及胶质瘢痕较其他3组都相对减少,而细胞静脉移植组和细胞局部移植组相近。以上结果表明静脉移植的人脐带间充质干细胞能迁移到脊髓损伤部位,对急性脊髓损伤产生修复作用,且效果与局部移植相似。该方式简单易行,避免二次损伤、感染等,为治疗脊髓损伤提供了一种新的细胞移植途径。BACKGROUND：Currently, human umbilical cord derived-mesenchymal stem cel s are mainly for local transplantation, which has some shortcomings, such as large trauma, bleeding, complications, that limit its widespread application in clinical practice. OBJECTIVE：To investigate the feasibility of intravenous transplantation of human umbilical cord derived-mesenchymal stem cel s for repair of spinal cord injury. METHODS：Eighty Wistar rats with spinal cord hitting were divided into five groups：blank control group with no transplantation （n=10）, DMEM local transplantation group （n=15）, DMEM intravenous transplantation group （n=15）, cel local transplantation group （n=20）, cel intravenous transplantation group （n=20）. The functional recovery of spinal cord injury was observed with Basso, Beattie and Bresnahan scores at regular time as wel as hematoxylin-eosin staining and immunohistochemistry staining. RESULTS AND CONCLUSION：During 1 day to 2 weeks after transplantation, there was no significant difference in the Basso, Beattie and Bresnahan scores between the five groups;within 4-12 weeks after transplantation, the Basso, Beattie and Bresnahan scores were significantly higher in the two cel transplantation groups than the other three groups, but there was no difference between these two cel transplantation groups （P〉0.05）. Histological observation showed that the number of voids and glial scars was less in the cel local transplantation group and cel intravenous transplantation group compared with the other three groups, and there was also no difference between the two cel transplantation groups. These results indicate that the intravenous transplantation of human umbilical cord derived-mesenchymal stem cel s is similar to the local transplantation in the repair of acute spinal cord injury, which is simple and avoids secondary injuries and various complications. It is recommended that this method provide a new approach for cel transplantation.

关 键 词：干细胞 移植 静脉移植 人脐带间充质干细胞 局部移植 脊髓损伤 

分 类 号：R394.2[医药卫生—医学遗传学]