人表皮生长因子受体2模拟表位的筛选  

Screening and identification of the minotops of human epidermal growth factor receptor 2

作　　者：李晓霞[1] 师建国[1] 陈果[1] 贺雪姣[1] 

机构地区：[1]第四军医大学基础医学部病理生理学教研室,陕西西安710032

出　　处：《现代肿瘤医学》2015年第12期1644-1646,共3页Journal of Modern Oncology

摘　　要：目的:从噬菌体12肽库中筛选出人表皮生长因子受体2(Her2)的抗原模拟表位。方法:以曲妥珠单抗为靶分子,在噬菌体12肽库中进行3轮淘选,以ELISA方法及竞争抑制实验鉴定阳性克隆,并对阳性克隆株进行测序。结果:经过3轮淘选,与曲妥珠单抗结合的噬菌体得到了有效富集,回收率从(2.00×10-8)%增加到(2.87×10-5)%,ELISA显示20个克隆中筛选获得了18个与曲妥珠单抗具有较高亲和性的阳性噬菌体,对阳性克隆测序获得两种氨基酸序列:HTSSLWHLFRST、VHWDFRQWWQPS。结论:噬菌体展示技术可成功筛选到表皮生长因子2模拟表位,为探索乳腺癌的防治研究创造了条件。Objective：To screen the minotops of Her2 from the phage display 12 peptide library. Methods：Used the Herceptin as the ligand to select the positive clones after 3 rounds of screening by the phage display 12 peptide li- brary. The positive clones were identified by enzyme linked immunosorbent assay（ELISA） and competitive inhibition assay. The identified positive clones were analyzed by DNA sequencing. Results：The phages were effectively enriched after 3 rounds of planning with the improvement of the recovery rate was from（2.00 × 10 ^- 8 ） % to （2.87 × 10^- 5 ） %. In 20 individual phages ,the 18 phages could combine the epidermal growth factor 2 monoclonal antibody. Two kinds of different sequences（ HTSSLWHLFRST, VHWDFRQWWQPS） were obtained. Conclusion：The Her2 related antigen epitope is obtained by phage display technology. The result provides a new approach for breast cancer therapy and vac- cine development.

关 键 词：表皮生长因子受体2 噬菌体肽库 模拟表位 

分 类 号：R730.53[医药卫生—肿瘤]