卧龙沙棘果实中多酚类成分的含量测定  

Determination of the polyphenols in berries of Hippophae rhamnoides subsp. wolongensis

作　　者：杜蕾蕾[1] 陈雏[2] 李亚真[3] 王雪彦[4] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137 [2]四川省中医药科学院,四川成都610041 [3]四川大学华西药学院,四川成都610041 [4]四川大学华西医院药剂科,四川成都610041

出　　处：《华西药学杂志》2015年第3期336-338,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81202908);四川省教育厅科研项目(No.14ZB0082)

摘　　要：目的测定卧龙沙棘果实中多酚类成分的含量。方法采用比色法测定总酚和总黄酮的含量;采用UPLC法测定槲皮素、山柰酚和异鼠李素的含量。结果 5批沙棘果实中总酚、总黄酮、槲皮素、山柰酚、异鼠李素的含量分别为29.80~38.80、17.60~27.40、0.38~2.01、0.17~2.00、0.49~4.53 mg·g^-1。结论卧龙沙棘果实中多酚类成分的含量较高,值得进一步研究与开发。OBJECTIVE To determine the polyphenols contents in berries of Hippophae rhamnoides subsp. wolongensis. METHODS The contents of total polyphenols and total flavonoids were measured by Colorimetry. Quercetin,kaempferol and isorhamnetin were quantified by UPLC. RESULTS The contents were found to be 29. 80-38. 80 mg·g^-1of total polyphenols,17. 60-27. 40 mg·g^-1of total flavonoids,0. 38-2. 01 mg·g^-1of quercetin,0. 17-2. 00 mg·g^-1of kaempferol and 0. 49-4. 53 mg·g^-1of isorhamnetin.CONCLUSION H. rhamnoides subsp. wolongensis,which the polyphenols are rich in the berries,has potential health value meriting further investigations.

关 键 词：卧龙沙棘 果实 多酚 黄酮 比色法 超高效液相色谱法 含量 测定 

分 类 号：R917[医药卫生—药物分析学]