双水相萃取双优红葡萄籽中的白藜芦醇  

Aqueous Two-phase Extraction of Resveratrol from Red Grape Seed

作　　者：曹雪杰 高明波 朴晶晶 廖颖玲 

机构地区：[1]大连民族大学生命科学学院,辽宁大连116600

出　　处：《广州化工》2015年第11期65-67,共3页GuangZhou Chemical Industry

基　　金：大连金州新区科技局项目(2012-A1-05);大连民族学院大学生创新创业训练计划项目(2014120260174);大连民族学院"太阳鸟"学生科研项目(tyn2014091)

摘　　要：葡萄酿酒过程中会产生大量的副产品,主要包括:葡萄籽,葡萄皮和葡萄茎;这些副产品约占葡萄总成分的20%。这些副产品是葡萄酿酒后的下脚料,一般被当做废弃物。白藜芦醇却主要存在于以上副产品中。其具有抗菌、抗癌、抗氧化、抑制血小板凝集和舒张血管、提高机体免疫力等特性。本实验利用双水相萃取的方法萃取"双优红"葡萄籽中的白藜芦醇,萃取过程绿色简洁,比传统有机溶剂萃取法有更好的萃取效果。从16种双水相体系中优选出最佳双水相体系乙醇/磷酸氢二钾。在单因素实验基础上,通过四因素水平对比实验优化白藜芦醇的萃取条件为:料液比为1∶15,温度为60℃,乙醇萃取浓度为70%,萃取时间为2.5 h,在此最佳工艺条件下白藜芦醇的萃取得量为0.484%。The wine making process produces a large quantity of grape secondary products, which include grape seeds, skins, and stems and so on. These byproducts comprise approximately 20% of the harvested grapes. All the byproducts are only scraps from the grape wine, generally treated as waste. Resveratrol, exists mainly in the above byproducts, owning a number of functions, such as antibacterial, anti-cancer, anti-oxidation function. It can also inhibit platelet aggregation, bring vasodilation and improve the body immunity. The method of aqueous two-phase extraction was used to extract the resveratrol in the grape Shuangyouhong seeds. The whole extraction is green and simple, which was far better than the traditional organic solvent extraction. Of the 16 kinds of aqueous two-phase systems, the best one of ethanol/ dipotassium phosphate system was optimized. On the basis of single factor experiment and the following four factors level contrast experiment, the extraction conditions of resveratrol were optimized as follows： 70% ethanol, solid to liquid ratio of 1：15, extraction for 2. 5 h at 60 ℃. The content of resveratrol in the Shuangyouhong grape seeds was 0. 484%.

关 键 词：白藜芦醇 萃取 双水相 乙醇/磷酸氢二钾 

分 类 号：TQ914.1[化学工程]