局部注射丹参酮Ⅱ-A对正畸过程中牙周膜VEGF表达的影响  

Effect of Local Injections of Tanshinone Ⅱ-A on VEGF Expression of Periodontal Membrane in Rabbit Orthodontic Tooth Movement

作　　者：杨夏晴 张军梅[1] 王薮馨 冯安琪 莫朝伦 

机构地区：[1]贵阳医学院,贵州贵阳550004 [2]贵阳医学院附院口腔科,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第5期467-470,共4页Journal of Guiyang Medical College

基　　金：贵阳市科技局基金[筑科合同(20141001)40号]

摘　　要：目的:研究局部注射丹参酮Ⅱ-A对正畸牙移动过程中牙周膜压力侧VEGF表达的影响。方法:选取20只兔,随机均分为第1、3、7,14和21天组,复制兔双侧下颌第一磨牙的正畸牙移动模型,以左侧下颌第一磨牙作为实验组,每日1次于近中牙龈黏膜下注射丹参酮Ⅱ-A磺酸钠注射液0.36 mg/d,右侧注射等量生理盐水作为对照组,分别于持续注射后1、3、7、14、21 d时麻醉处死动物,切取双侧下颌第一磨牙及周围组织制成牙周组织切片,观察VEGF的相对表达量,测量下颌第一磨牙移动距离。结果:除第1天组外,其余各个时间点实验组牙移动距离大于对照组,差异有统计学意义(P<0.05);第3、7、14、21天各组VEGF平均染色强度高于第1天,且实验组高于对照组,差异有统计学意义(P<0.05),第7天实验组VEGF平均染色强度最高,差异有统计学意义(P<0.01)。结论:丹参酮Ⅱ-A促进牙移动过程中血管内皮细胞VEGF表达,增加新生血管的数量,对加速正畸牙移动有一定的作用。Objective： To investigate the effect of local injection of tanshinone Ⅱ-A on vascular endothelial growth factor（ VEGF） expression of periodontal membrane in orthodontic tooth movement.Methods： Twenty rabbits were randomly divided into 5 groups and named as 1stday group,3rdday group,7thday group,14 thday group and 21 stday group with four rabbits in each. Copying the orthodontic tooth movement model of the bilaterally first molar of rabbits to measure the mesial movement distance of the mandibular first molar on the plaster model at different time. 0. 36 mg / d of tanshinoneⅡ-A sulfonate was injected into the mesial gingival mucosa of the left first molar once a day,which served as the experimental group,the same amount of normal saline（ NS） was injected into the right molar served as the control group. After injecting for 1,3,7,14 and 21 days,the rabbits were put to anesthesia death and cut out the organization to make slice. The periodontal tissue change and the expression of VEGF were observed. Results： Except for the 1st day group,the tooth movement distance of the experimental group were larger than that of control group and the difference was statistically significant（ P〈0. 05）. On 3^rd,7^th,14 ^th and 21^st day,all groups ＇ VEGF average color intensity were higher than that of the control group and the difference was statistically significant（ P〈0. 05）. Conclusions： The experiment indicates that tanshinone Ⅱ-A can effectively increase the VEGF expression and the number of vascular,and have certain effect to accelerate the orthodontic tooth movement.

关 键 词：丹参酮II-A 兔 血管内皮生长因子 正畸学 牙移动 

分 类 号：R783.5[医药卫生—口腔医学]