4种纳米羟磷灰石复合材料对粪肠球菌抗菌性能的初步研究  

Study on the antibacterial activity of four kinds of nano-hydroxyapatite composites against Enterococcus faecalis

作　　者：刘奕[1] 周荣静[2] 吴红崑[3] 

机构地区：[1]四川省医学科学院.四川省人民医院口腔科,成都610072 [2]聊城市人民医院口腔科,聊城252000 [3]口腔疾病研究国家重点实验室华西口腔医院特需门诊(四川大学),成都610041

出　　处：《华西口腔医学杂志》2015年第3期301-305,共5页West China Journal of Stomatology

基　　金：四川省科技应用基础计划基金资助项目(2008JY0037)

摘　　要：目的筛选一种对粪肠球菌有抑菌效能的纳米羟磷灰石复合材料。方法选用载银纳米羟磷灰石(Ag/n HA)、载钇纳米羟磷灰石(Yi/n HA)、载铈纳米羟磷灰石(Ce/n HA)、普通纳米羟磷灰石(n HA)为实验材料,分别采用琼脂扩散法和琼脂稀释法,通过计量不同实验材料对粪肠球菌的抑菌环直径和最小抑菌浓度(MIC),评价4种纳米复合材料对粪肠球菌的影响。结果琼脂扩散法显示Ag/n HA周围出现明显的抑菌环,而Yi/n HA、Ce/n HA、n HA周围均无抑菌环。琼脂稀释法显示Ag/n HA对粪肠球菌的MIC为1.0 g·L-1;其他材料即使浓度高达32.0 g·L-1也未表现出抑制粪肠球菌的作用。结论 Ag/n HA对浮游状态的粪肠球菌有较好的抑制作用,而Yi/n HA、Ce/n HA、n HA在实验浓度范围内均无明显地抑制粪肠球菌的作用。ObjectiveThis study aims to compare and determine a kind of nano-hydroxyapatite composite material with good antibacterial efficacy on Enterococcus faecalis（E. faecalis） in vitro. Methods We investigated the antimicrobial acti-vity of four kinds of nano-hydroxyapatite composites, namely, silver/hydroxyapatite composite nanoparticles （Ag/nHA）, yttrium/hydroxyapatite composite nanoparticles （Yi/nHA）, cerium/hydroxyapatite composite nanoparticles （Ce/nHA）, and hydroxyapatite nanoparticles （nHA）, against E. faecalis in vitro using the agar diffusion and broth dilution method by mea-suring the growth inhibition zone and the minimum inhibitory concentration （MIC）, respectively. Results The agar diffu-sion test results showed that Ag/nHA displayed an obvious growth inhibition zone, whereas Yi/nHA, Ce/nHA, and nHA showed no influence on E. faecalis. The MIC value of Ag/nHA was 1.0 g·L^-1, and the three other materials had no effect on E. faecalis even at the high concentration of 32.0 g·L^-1. Conclusion Ag/nHA display a potential antimicrobial efficacy to planktonic E. faecalis. Whereas, the three other kinds of nano-hydroxyapatite composites （Yi/nHA, Ce/nHA, nHA） show no influence.

关 键 词：纳米羟磷灰石 粪肠球菌 抑菌性能 

分 类 号：R783.1[医药卫生—口腔医学]