酶解与离心分相结晶协同制备抗性淀粉工艺研究  

Resistant Starch Prepared by Enzymatic Hydrolysis Cooperated with Phase Separation by Centrifugation

作　　者：冯军伟[1] 黄继红[1,2] 苏雪锋[1] 游倩[1] 侯银臣 王文[1] 杨铭乾 

机构地区：[1]河南工业大学生物工程学院,郑州450001 [2]郑州市中食农产品加工研究院,郑州450001

出　　处：《中国粮油学报》2015年第6期49-52,66,共5页Journal of the Chinese Cereals and Oils Association

基　　金：河南工业大学横向项目(151260);河南省产学研合作项目(12210700003)

摘　　要：以抗性淀粉含量和产品收率为评价指标,对耐高温α-淀粉酶添加量单因素优化分析和离心因素对照分析,得到早籼米抗性淀粉的最佳制备工艺条件:耐高温α-淀粉酶量为1.20 U/g,脱支液4 000 r/min离心15 min,重相和轻相分别结晶,经洗糖、干燥后得重相抗性淀粉质量分数可达30.69%,收率20.35%,轻相抗性淀粉质量分数可达45.82%,收率67.75%。2种产品对原淀粉总收率88.10%。并分析抗性淀粉碘吸收曲线和聚合度,表明相近聚合度(一定范围内)的直链淀粉分子越集中越利于结晶形成抗性淀粉。该工艺验证并充实了抗性淀粉形成机理假说:本质是直链淀粉分子的结晶,基本满足工业化生产高含量抗性淀粉产品的要求。Efficient preparation of resistant starch （RS） from early indica rice is studied to achieve the appreciation of the cheap rice. Evaluating the α-amylase amounts and comparison of the resistant starch in heavy portion （HRS） and resistant starch in light portion （LRS） by the content and yield of resistant starch, the optimal preparation techniques were as follows： α-amylase which has a optimal amount of 1.20 U/g, centrifuging at 4000 r/min for 15 min, crystallizing heavy and light resistant starch respectively, dissolving the soluble oligosaccharides, drying. The content of resistant starch in light resistant starch was 30.65% （w/w） with a yield 20.35%, while the content of resistant starch in light resistant starch was 45.82% with a yield 67.75%. The total yield of two products was 88.10%. The analysis of iodine absorption curve and degree of polymerization （DP） of two products shows that the more amylose molecules with similar DP, the more formation of resistant starch. Basically meeting the requirements of industrial production for products rich in resistant starch, this preparation technique verified and developed the hypothesis of RS formation mechanism.

关 键 词：早籼米抗性淀粉 离心分相 RS含量与收率评价 聚合度 

分 类 号：Q814.9[生物学—生物工程]