miR-199在对顺铂敏感性不同的卵巢癌细胞株中的表达及其意义  

The expression and significance of miR-199 in ovarian cancer cell lines

作　　者：柳长青[1,2] 徐汉杰[1,3] 周颖[3] 赵卫东[1,2] 

机构地区：[1]安徽医科大学附属省立医院安徽省立医院,合肥230001 [2]安徽省肿瘤医院 [3]安徽省分子医学重点实验室

出　　处：《中国临床保健杂志》2015年第3期290-293,I0001,共5页Chinese Journal of Clinical Healthcare

基　　金：国家自然科学基金(81272881);安徽省科技攻关计划项目(08010302101)

摘　　要：目的探讨miR-199在卵巢癌细胞株中的表达,寻找卵巢癌化疗敏感性的指标。方法组学分析找出在顺铂敏感性不同的卵巢癌细胞株,miR-199的表达量差异较为显著,qRT-PCR和western blot方法检测瞬时转染miR199 mimic/antagomir后的卵巢癌细胞株,P63表达水平发生显著变化。结果 miR-199在对顺铂耐药的ES-2细胞中表达水平高于在对顺铂耐药的SKOV3细胞中的表达,miR-199的下游蛋白P63的表达也有差异。结论 miR-199与P63在一定程度上可以预测卵巢癌顺铂化疗敏感性,miR-199可能是通过其下游靶基因P63发挥其在卵巢癌顺铂化疗敏感性中的作用。Objective In this study,we aimed to detect the expression of miR -199 and searched for chemo-therapy sensitivity of indicators in ovarian cancer cell lines and.Methods Omics analysis had found that in ovarian cancer cell lines which had different sensitivities to cisplatin,the difference of expression of miR -199 is significant. Then used specificity methylation PCR and western blot to verify the expression level of P63 gene in ovarian cancer cell lines of the transient transfection miR199 mimic /antagomir.Results The expression level of miR-199 in ES-2 is much higher than in the expression of SKOV3 cells.The expression of p63 is negatively correlated with the expression of miR-199.Conclusions miR-199 and P63 may predict the sensitivity to cisplatin chemotherapy to some extent. miR-199 may effect the sensitivity to cisplatin chemotherapy of ovarian cancer by regulating its downstream target genes P63.

关 键 词：卵巢肿瘤 抗药性 肿瘤 顺铂 紫杉酚 氟尿嘧啶 

分 类 号：R737.31[医药卫生—肿瘤]