唐氏综合征胎儿脐带血与外周血microRNA差异表达研究  

Research on micro RNA expression profi les in Down syndrome umbilical cord blood and peripheral blood

作　　者：林华[1] 眭维国[1] 陈洁晶[1] 谭秋培[1] 郭丽[1] 李凤艳[1] 李武县 戴勇 

机构地区：[1]中国人民解放军第一八一医院肾脏科广西代谢性疾病研究重点实验室,广西桂林541002 [2]深圳市人民医院临床医学研究中心,广东深圳518020

出　　处：《中国优生与遗传杂志》2015年第5期15-19,共5页Chinese Journal of Birth Health & Heredity

基　　金：广西重点实验室建设项目计划任务书资助项目(13-051-31);桂林市科技计划科技攻关资助项目(20130120-21)

摘　　要：目的检测唐氏综合征(Down syndrome,DS)脐带血与外周血单个核细胞中micro RNA差异表达图谱,进行比较分析。方法 Illumina检测脐带血与外周血中micro RNA的表达水平,比较两组间的差异表达,并进行靶基因预测与基因功能注释。结果脐带血与外周血显著差异表达的micro RNA分别有344和253种,分析可知,外周血表达上调的mi RNA增加了179种,下调的减少了270种。GO分析有5个生物学过程在预测的靶基因中被有意义的富集(P<0.001)。结论 DS脐带血与外周血micro RNA的表达差异显著,可能与胎儿生长发育过程中免疫系统缺陷,智力低下等症状的发生,发展相关。Objective： To detect the microRNA differentially expression profiles in mononuclear cells from Down syndrome umbilical cord blood and peripheral blood, and comparative analysis. Methods： Illumina was used to detect the microRNA differentially expression in cord blood and peripheral blood samples, and the target gene biological function was analyzed. Results： A total of 344 and 253 miRNAs were found significantly dysregulated expression in the DS cord blood and peripheral blood compared to the controls, respectively. A total of 179 and 270 miRNAs were found significantly expression in the DS peripheral blood mononuclear cells compared to the cord blood. A total of 5 GO （gene ontology） terms in biological processes were significantly enriched （P〈0.001） . Conclusion： These results suggested that there is a significant difference between the expression patterns of miRNAs in the DS peripheral blood group and cord blood group, which may be related to the immune deficiency, mental retardation development proceeding of DS fetus.

关 键 词：唐氏综合征 微小RNA 脐带血 外周血 Illumina技术 

分 类 号：R714.5[医药卫生—妇产科学]