YKL-40和IL-6在结肠癌组织中表达变化的研究  

Experimental research of expression of YKL- 40 and IL- 6 in colorectal cancer tissues

作　　者：杨阳[1] 杨晶晶[2] 宁宁[3] 丁毅[1] 金伟森[1] 

机构地区：[1]安徽医科大学武警总医院临床学院,合肥230032 [2]安徽医科大学第二附属医院药学部,合肥230601 [3]中国人民解放军总医院普外科,北京100039

出　　处：《安徽医科大学学报》2015年第6期727-730,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81201688)

摘　　要：目的探讨结肠癌组织中人软骨糖蛋白39(YKL-40)和白细胞介素-6(IL-6)蛋白的表达变化及其临床意义。方法采用Western blot、qRT-PCR法检测30例接受根治性手术治疗的结肠癌患者的结肠癌组织标本及结肠癌旁组织中YKL-40和IL-6的表达变化。分析患者YKL-40和IL-6的表达与结肠癌的组织分化程度等临床病理学特征的关系。结果 Western blot实验结果显示结肠癌组织中YKL-40和IL-6蛋白表达明显高于结肠癌旁组织(P<0.05),qRT-PCR结果表明结肠癌组织中YKL-40和IL-6mRNA表达明显高于结肠癌旁组织(P<0.05)。结肠癌组织中YKL-40与IL-6表达呈正相关性(r=0.812,P<0.05)。此外,YKL-40和IL-6表达与TNM分期、分化程度相关(P<0.05)。结论 YKL-40和IL-6在结肠癌组织中呈高表达,可能与结肠癌的发生、发展有关。Objective To observe the changes of expression of cartilage glycoprotein-39（YKL-40）and interleukin＆nbsp;6（IL-6）in colon cancer tissues. Methods Colon tissues were isolated from 30 colon cancer patients who had re-ceived radical surgeries normal persons. Western blot and qRT-PCR were used to detect the expression of YKL-40 and IL-6. Analyzing the relationship between the expression of YKL-40 and tumor differentiation,the depth of tumor invasion,lymph node metastases and other clinical pathological characteristics. Results Compared with the colon tissue beside tumor tissue,Western blot showed that the protein expressions of YKL-40 and IL-6 were signifi-cantly increased in the model group（P 〈 0. 05）. qRT-PCR found that the mRNA expression of YKL-40 and IL-6 are significantly increased in the model group（P 〈 0. 05）. The intensive expression of YKL-40 was positively cor-related to TNM stage and pathologic grade（P 〈 0. 05）. Conclusion The expression of YKL-40 and IL-6 in colon cancer is high and this may have relationship with tumor progression.

关 键 词：结肠癌 YKL-40 IL-6 

分 类 号：R735.35[医药卫生—肿瘤]